中国肿瘤生物治疗杂志2018，Vol.25Issue(4)：340-345,6.DOI:10.3872/j.issn.1007-385x.2018.04.004
积雪草酸通过抑制耐药相关蛋白表达增强U87MG胶质瘤细胞对紫杉醇的敏感性
Asiatic acid enhances the chemosensitivity of U87MG glioma cells to paclitaxel through inhibiting the expression of drug resistance related proteins
张磊 1陈磊 2陈杰 1杨晶晶1
作者信息
- 1. 济南市市中区人民医院儿科,山东济南250023
- 2. 济南市市中区人民医院肿瘤科,山东济南250023
- 折叠
摘要
Abstract
Objective:To explore the inhibitive effect of asiatic acid (AA) on paclitaxel (PTX)-resistant glioma cells and its possible mechanism.Methods:The effects of AA on the proliferation and apoptosis of glioblastoma U87MG cells were detected by CCK-8 assay,Real-time quantitative polymerase chain reaction (qPCR) and Western blotting.The drug-resistant glioma cell line PR-U87MG was established by culturing the cells in concentration-increasing PTX.With U87MG cells as control,the PTX-resistance of PR-U87MG cells was confirmed using CCK-8 assay,and the mRNA and protein levels of MDR1 and LRP were measured with qPCR and western blotting.PR-U87MG cells were treated with AA,PTX or AA+PTX,and then the cell viability and apoptosis of each group were measured with CCK-8 assay,qPCR and Western blotting.Results:PTX-resistant PR-U87MG cell line was successfully established.AA inhibited the viability of U87MG and PR-U87MG cells in a dose-dependent manner (P＜0.01) and significantly promoted their apoptosis (P＜0.01).Compared with the group treated with AA or PTX alone,the group treated with the combination of AA and PTX had significantly decreased protein levels of PARP1 (P＜0.01),drug-resistant related proteins (Pgp-1 and LRP [lung resistance protein],all P＜ 0.01),and markedly increased caspase 3 (P＜0.01).Conclusion:AA could effectively enhance the sensitivity of U87MG cells to PTX,and the mechanism may be related to the suppressed expression of drug effiux-associated proteins Pgp-1 and LRP.关键词
积雪草酸/胶质瘤/耐药性/化药敏感性/紫杉醇/U87MGKey words
asiatic acid/glioma/drug resistance/chemosensitivity/paclitaxel/U87MG分类
医药卫生引用本文复制引用
张磊,陈磊,陈杰,杨晶晶..积雪草酸通过抑制耐药相关蛋白表达增强U87MG胶质瘤细胞对紫杉醇的敏感性[J].中国肿瘤生物治疗杂志,2018,25(4):340-345,6.
