中国肿瘤生物治疗杂志2018，Vol.25Issue(4)：346-350,5.DOI:10.3872/j.issn.1007-385x.2018.04.005
锌转运体1基因在脑胶质瘤组织中的表达及其对U87细胞增殖、迁移和侵袭能力的影响
Expression of zinc transporter 1 gene in brain glioma tissues and its effects on proliferation, migration and invasion of glioma U87 cell line
摘要
Abstract
Objective:To detect the expression of zinc transporter 1 (ZnT1) gene in glioma tissue,and to explore its effect on the proliferation,migration and invasion of U87 cells.Methods:From October 2015 to January 2017,20 patients with glioma,who received no chemoradiotherapy before operation,were collected from Department of Neurosurgery,the First Affiliated Hospital of China Medical University.The protein and mRNA content of ZnT1 in glioma tissues and adjacent tissues were detected by Western blotting and Realtime PCR,respectively.ZnT1 and si-ZnT1 plasmids were transfected into glioma U87 cell line respectively to construct ZnT1 over-expression U87 cell line and ZnT1 knockdown U87 cell line.The effects of ZnT1 on proliferation,migration and invasion of U87 cells were detected by MTT and transwell assay.Results:Both mRNA and protein expressions of ZnT1 in glioma tissues was significantly higher than those in adjacent tissues (all P＜0.05).U87 cell lines with ZnT1 over-expression and knockdown were successfully constructed.Compared with the control group and empty plasmid control group,the proliferation (0.54±0.01 vs 0.45±0.04,0.43±0.03,P＜0.01),invasion and migration (all P＜0.05) of U87 cells with ZnT1 over-expression were significantly increased at 12 h after transfection;however,the proliferation (0.37±0.03 vs 0.45±0.01,0.44±0.03,P＜0.01),invasion and migration (all P＜0.05) of U87 cells with ZnT1 knockdown were decreased significantly.Conclusion:ZnT1 was highly expressed in glioma tissues,and promoted the proliferation,migration and invasion of glioma U87 cells.关键词
锌转运体1基因/胶质瘤/U87细胞/增殖/迁移/侵袭Key words
zinc transporter 1 gene/glioma/U87 cell/proliferation/migration/invasion分类
医药卫生引用本文复制引用
王维,张路阳,张东勇,仇波,王运杰,包义君..锌转运体1基因在脑胶质瘤组织中的表达及其对U87细胞增殖、迁移和侵袭能力的影响[J].中国肿瘤生物治疗杂志,2018,25(4):346-350,5.基金项目
教育部留学回国人员科研启动基金资助项目(教外司留[2013]1792号) （教外司留[2013]1792号）
辽宁省自然科学基金面上项目资助(No.2015020460) （No.2015020460）
中国博士后科学基金第59批面上项目资助(No.2016M590240) （No.2016M590240）
沈阳市科技计划项目资助(No.17-230-9-13) （No.17-230-9-13）
中国医科大学附属第一医院科学研究基金助(No.FSFH201722). Project supported by the Overseas Returnees Research Foundation of Ministry of Education (No.[2013]1792) （No.FSFH201722）
the Natural Science Foundation of Liaoning Province (No.2015020460) （No.2015020460）
the Chinese Postdoctoral Science Foundation (No.2016M590240) （No.2016M590240）
the Science And Technology Project of Shenyang City (No.17-230-9-13) （No.17-230-9-13）
and the Scientific Research Foundation of the First Affiliated Hospital of China Medical University (No.FSFH201722) （No.FSFH201722）
