中国肿瘤生物治疗杂志2018，Vol.25Issue(4)：351-356,6.DOI:10.3872/j.issn.1007-385x.2018.04.006
FOXD1在胶质瘤组织中的表达及其与患者预后的关系
Expression of FOXD1 in glioma tissues and its correlation with prognosis of patients
摘要
Abstract
Objective:To evaluate the expression level of FOXD1 in glioma tissues of different grades,and to investigate the correlation between the expression of FOXD1 and the prognosis of glioma patients.Methods:The tumor tissues were collected from 40 glioma patients,who received surgical treatment in the neurosurgery department of the First Hospital of China Medical University from September 2014 to February 2015;Seven non-tumor tissues obtained from patients underwent internal decompression for traumatic brain injury were used as controls.The FOXD1 expression in glioma and non-tumor brain tissues was analyzed by qRT-PCR and IHC,and the correlations between clinical pathological features of glioma patients and FOXD1 expression level were analyzed.Furthermore,the Kaplan-Meier method was used to analyze the relationship between FOXD1 expression and survival time of patients.In addition,the expression of FOXD1 in glioma tissues and its relationship with patients' prognosis were confirmed by the data from GEO (GSE4290,GSE2223) and Rembrandt database.Results:qRT-PCR showed that the FOXD1 mRNA expression in glioma tissues of WHO grade Ⅳ was significantly higher than that of non-tumor brain tissues and glioma tissues of WHO grade Ⅱ (P＜0.01).German immunohistochemical score (GIS) was used to evaluate the immunohistochemical staining intensity,and the relationship between FOXD1 expression and clinical pathological features was analyzed.The results showed that FOXD1 in glioma tissues was related to WHO phathological grade level (x2=11.73,P＜0.01).There was statistically significant difference between the survival time of FOXD1 high expression group and FOXD1 low expression group (P=0.043).The data from GEO data base (GSE4290,GSE2223) and Rembrandt datasets showed that glioma tissues have a higher FOXD1 mRNA expression level than normal brain tissues,and the elevated expression of FOXD1 mRNA was negatively associated with the survival time of glioma patients.Conclusion:FOXD1 was highly expressed in glioma tissues,and the expression level of FOXD1 was increased as the pathological grade increases.The elevated expression of FOXD1 was related with the poor survival of glioma patients.关键词
胶质瘤/叉头框基因/生存率/预后Key words
glioma/forkhead box (FOX) gene/survival rate/prognosis分类
医药卫生引用本文复制引用
姜泽宇,陆威成,郭宗泽,程鹏..FOXD1在胶质瘤组织中的表达及其与患者预后的关系[J].中国肿瘤生物治疗杂志,2018,25(4):351-356,6.基金项目
辽宁省科学技术计划资助项目(No.2012225014). Project supported by the Science and Technology Plan Projects of Liaoning Province (No.2012225014) （No.2012225014）
