中国肿瘤生物治疗杂志2018，Vol.25Issue(4)：394-400,7.DOI:10.3872/j.issn.1007-385x.2018.04.013
金纳米星负载二氢卟吩e6对肺癌A549细胞的光动力效应
Photodynamic effects of gold nanostars loading chlorin e6 on lung cancer A549 cells
摘要
Abstract
Objective:To prepare GNS (gold nanostars) loading photosensitizer chlorin e6 (Ce6) and to investigate its photodynamic effects on lung cancer A549 cells.Methods:GNS was firstly modified by SH-PEG-NH2 and then mixed with Ce6 and shaken overnight to prepare GNS-PEG@Ce6,which had photodynamic therapy effects.The characterization,morphology and encapsulation rate were detected.The difference between the phagocytosis of Ce6 and GNS-PEG@Ce6 by A549 cells were observed with a Leical TCS SP8 confocal laser scanning microscope.MTT assay was used to examine the inhibitory effect of GNS-PEG@Ce6 on the proliferation ofA549 cells while FCM was used to detect the effect of probe GNS-PEG@Ce6 on the apoptosis ofA549 cells.Results:The particle size of the GNS-PEG@Ce6 was about 100 nm.The prepared GNS-PEG@Ce6 nanoparticles exhibited good dispersion and stability and the encapsulation rate of Ce6 was about 50％.GNS-PEG@Ce6 entered the cells by endocytosis and mainly distributed in the cytoplasm;compared with Ce6,GNS-PEG@Ce6 could enter the cells more effectively.The proliferation-suppression effect of GNS-PEG@Ce6 on A549 cells was significantly stronger than that of Ce6 (P＜0.05).The results of flow cytometry showed that the probe exhibited strong apoptotic effect on A549 cells.Conclusion:GNS,as the drug carrier,could effectively increase the Ce6 uptake efficacy in A549 cells,thus further enhancing the killing effects of Ce6 on lung cancer A549 cells.关键词
金纳米星/二氢卟吩e6/肺癌/A549细胞/光动力治疗Key words
gold nanostars/Chlorin e6/lung cancer/A549 cells/photodynamic therapy分类
医药卫生引用本文复制引用
李晨露,夏芳芳,章阿敏,崔大祥..金纳米星负载二氢卟吩e6对肺癌A549细胞的光动力效应[J].中国肿瘤生物治疗杂志,2018,25(4):394-400,7.基金项目
国家重点研发计划资助项目(No.2017YFA0205301,No.2015CB931802) （No.2017YFA0205301,No.2015CB931802）
上海市经信委资助项目(No.XC-ZXSJ-02-2016-05). Project supported by the Funding Project of National Key Research and Development Program (No.2017YFA0205301,No.2015CB931802),and the Funding Project of Shanghai Economic and Information Committee (No.XC-ZXSJ-02-2016-05) （No.XC-ZXSJ-02-2016-05）
