制冷学报2018，Vol.39Issue(3)：126-134,9.DOI:10.3969/j.issn.0253-4339.2018.03.126
胞内冰形成机理研究进展
Review of the Mechanism of Intracellular Ice Formation
摘要
Abstract
One of the important challenges in developing successful cryopreservation protocols for biological samples relies on avoiding the freezing damage imposed by the intracellular ice formation ( IIF) . Experimental studies have shown that the formation of a traceable quan-tity of IIF is lethal. This complex phenomenon is affected by various factors including the cooling rate, water transport, cell membrane in-tegrity, extracellular ice, and cryoprotectant. The main goal of this work is to review the four classical intracellular ice formation theories that have been previously proposed:1) external ice grows through pores in the cell membrane and catalyzes the IIF, 2) external ice cau-ses a conformational change in the cell membrane, transforming it into an effective heterogeneous form and causing IIF, 3) the IIF is cata-lyzed heterogeneously through intracellular structures or homogeneously, and 4) the IIF is a result of plasma membrane damage. The latest experimental observations support the idea that the contact between the external ice and cell membrane leads directly or indirectly to the formation of intracellular ice. Aquaporins are likely a key factor affecting the IIF. The use of an aquaporin inhibitor and a gene knockout to study the IIF is also proposed in this article.关键词
低温保存/低温损伤/胞内冰/冰晶成核/水通道蛋白Key words
cryopreservation/cryoinjury/intracellular ice/ice nucleation/aquaporin分类
医药卫生引用本文复制引用
刘威,郭明伟,郭治宇,刘宝林..胞内冰形成机理研究进展[J].制冷学报,2018,39(3):126-134,9.基金项目
国家自然科学基金( 51776130 )资助项目. ( The project was supported by the National Natural Science Foundation of China ( No. 51776130). ) 本文受上海东方学者跟踪计划项目的资助. ( The project was supported by the Project Tracing Program of Oriental Schol-ars. ) （ 51776130 ）
