作物学报2018，Vol.44Issue(6)：824-835,12.DOI:10.3724/SP.J.1006.2018.00824
甘蔗磷脂酰肌醇转运蛋白基因ScSEC14响应干旱和盐胁迫
A Sugarcane Phosphatidylinositol Transfer Protein Gene ScSEC14 Responds to Drought and Salt Stresses
摘要
Abstract
Sec14-like phosphatidylinositol transfer proteins are present in all eukaryotic genomes and involved in a variety of biological activities, such as metabolism of inositol phosphate, membrane transportation, polar growth, signal transduction and stress responses. The responses of Sec14-like gene to drought and salt stresses have not been reported in sugarcane. In this study, a SEC14 gene sequence was obtained in the sugarcane transcription database infected by Sporisorium scitamineum, and the full length cDNA sequence of a sugarcane SEC14 gene was obtained by RT-PCR technology and named as ScSEC14 (GenBank accession number: MG571103). Bioinformatics analysis showed a full length of 1617 bp in ScSEC14 gene containing a complete open reading frame of 1008 bp and encoding 335 amino acid residues. ScSEC14 is an unstable hydrophilic protein with no signal peptide. The secondary structure of ScSEC14 protein is mostly α-helices, with a typical SEC14 domain and a CRAL_TRIO_N domain. Phylogenetic tree analysis showed that ScSEC14 belonged to SSH (soybean Sec14 homolog group) subfamily of Sec14-like protein family. Subcellular localization experiment showed that ScSEC14 protein was mainly localized in the plasma membrane. Real-time quantitative PCR analysis showed that ScSEC14 gene was constitutively expressed in sugarcane, with the lowest expression level in skin, and the highest in leaf, which was 4.9 times of that in skin. The expression of ScSEC14 gene was up-regulated under the stresses of PEG, NaCl, CaCl2 and salicylic acid (SA). We speculate that ScSEC14 plays an important role in response to drought and salt stresses, and may be involved in the stress response signaling pathway mediated by Ca2+ and SA.关键词
甘蔗/Sec14-like磷脂酰肌醇转运蛋白/ScSEC14/干旱/盐胁迫Key words
sugarcane/Sec14-like phosphatidylinositol transfer protein/ScSEC14/drought/salt stress引用本文复制引用
毛花英,苏亚春,阙友雄,刘峰,苏炜华,黄宁,凌辉,张旭,王文举,李聪娜,汤翰臣..甘蔗磷脂酰肌醇转运蛋白基因ScSEC14响应干旱和盐胁迫[J].作物学报,2018,44(6):824-835,12.基金项目
本研究由国家自然科学基金项目(31671752), 福建省杰出青年基金项目(2015J06006)和国家农业产业技术体系建设专项(CARS-17)资助. This study was supported by the National Natural Science Foundation of China (31671752), the Natural Science Foundation of Fujian Pro-vince for Distinguished Young Scholars (2015J06006), and the China Agriculture Research System (CARS-17). （31671752）
