作物学报2018，Vol.44Issue(6)：836-843,8.DOI:10.3724/SP.J.1006.2018.00836
CIMMYT小麦种质C615抗叶锈病QTL分析
Mapping QTLs against Leaf Rust in CIMMYT Wheat C615
李玉玲 1蒋正宁 2胡文静 2李东升 2程婧晔 3裔新 1程晓明 2吴荣林 2程顺和1
作者信息
- 1. 南京农业大学,江苏南京 210095
- 2. 江苏里下河地区农业科学研究所/国家小麦改良中心扬州分中心,江苏扬州 225007
- 3. 扬州大学农学院,江苏扬州 225009
- 折叠
摘要
Abstract
Leaf rust, caused by Puccinia triticina, is an important wheat disease. The introduced wheat line C615 shows adult resistance to leaf rust, but its mechanism is not clear. In this study, the quantitative trait loci (QTLs) for leaf rust resistance were identified and located in a linkage map constructed with 337 SSR markers using a recombinant inbred line (RIL) population (112 lines of the F2:7 generation) derived from CIMMYT wheat C615 (resistant) and Ningmai 18 (highly susceptible). Final disease severity (FDS) of leaf rust was evaluated in 2016 and 2017. Five QTLs were detected on chromosomes 1BL, 2DS, 3BS, 4DL, and 6BS, designated QLr.njau-1BL, QLr.njau-2DS, QLr.njau-3BS, QLr.njau-4DL, and QLr.njau-6BS, respectively. All the resistance alleles were contributed by the resistant parent C615, except for QLr.njau-2DS. Three QTLs, QLr.njau-1BL, QLr.njau-3BS, and QLr.njau-4DL, were detected in both years, which explained phenotypic variance by 10.1%–15.7%, 10.9%–13.5%, and 8.2%–9.0%, respectively. The remaining two QTLs were detected in one year with smaller contributions (6.2% and 9.2%,respectively). QLr.njau-1BL and QLr.njau-4DL were located in the same regions of the known resistance genes Lr46 and Lr67,respectively. QLr.njau-3BS in the marker interval Xbarc102–Xwmc623 is probably a novel QTL for leaf rust resistance. Fifteen BC4F5 lines derived from the cross of C615/Yangmai 13 (recurrent parent) were genotyped for leaf rust resistance using seven SSR markers closely linked to the QTLs from C615. All the 15 lines had QTLs donated by C615 and three of them pyramided four QTLs, indicating that C615 can serve as an elite donor parent in wheat breeding aiming at high-yield and high-resistance to leaf rust. These results provide a basis of marker-assisted selection and providing breeding materials.关键词
小麦/叶锈病/QTL/标记辅助选择育种Key words
wheat (Triticum aestivum L.)/leaf rust/QTL mapping/marker-assisted selection引用本文复制引用
李玉玲,蒋正宁,胡文静,李东升,程婧晔,裔新,程晓明,吴荣林,程顺和..CIMMYT小麦种质C615抗叶锈病QTL分析[J].作物学报,2018,44(6):836-843,8.
