作物学报2018，Vol.44Issue(6)：852-858,7.DOI:10.3724/SP.J.1006.2018.00852
大豆籽粒硬实加性和上位性QTL定位
Mapping Main-effect and Epistatic QTL for Hard Seededness in Soybean
摘要
Abstract
Hardness is a common characteristic of plant seeds, which is an important quantitative trait affecting germination rate, viability and storage life, and also processing quality of soybean seeds. In this study QTL analysis of the additive and epistatic interaction was used to reveal the important loci and effects controlling soybean hard seededness, and to provide theoretical basis for further analysis of the complex genetic mechanism of hard seededness. F6:8 and F6:9 populations of 186 recombinant inbred lines (RIL) derived from a cross of Jidou 12 and native variety Heidou (ZDD03651) were used to determine the additive QTLs for hard seededness in different years by the composite interval mapping (CIM) method in WinQTL Cartographer V. 2.5 software. The inclusive complete interval mapping (ICIM) method in IciMapping 4.1 software was used for analysing the interaction of additive and epistatic QTLs for hard seededness. Three QTLs for hard seededness were identified on Chr. 02, Chr. 06, and Chr. 14, respectively, with the genetic contribution rate of 5.54%–12.94%. Four pairs of epistatic interaction QTLs were detected on Chr. 02, Chr. 06, Chr. 09, Chr. 12, and Chr. 14, respectively, with explained 2.53%–3.47% of the phenotypic variation. The QTLs of additive and epistatic interactions were also detected in the hard seeds of soybean, and the epistasis was performed between the main effect QTLs or between the main effect QTL and the non-main effect QTL. The results indecate that the epistatic interaction effect plays an important role in the genetic basis of hard seededness of soybean.关键词
大豆/硬实/QTL/上位性互作Key words
soybean/hard seededness/QTL/epistasis引用本文复制引用
艾丽娟,陈强,杨春燕,闫龙,王凤敏,葛荣朝,张孟臣..大豆籽粒硬实加性和上位性QTL定位[J].作物学报,2018,44(6):852-858,7.基金项目
本研究由国家重点研发计划项目(2016YFD0100201),国家现代农业产业技术体系建设专项(CARS-004-PS06)和河北省重点研发计划(16227516D)资助.ThisstudywassupportedbytheNationalKeyResearchandDevelopmentProgramofChina(2016YFD0100201),theChinaAgricultureResearchSystem(CARS-004-PS06),andKeyResearchandDevelopmentProjectsofHebeiProvence(16227516D). （2016YFD0100201）
