作物学报2018，Vol.44Issue(6)：867-875,9.DOI:10.3724/SP.J.1006.2018.00867
四个抗豆象绿豆品种的胰蛋白酶抑制剂稳定性
Stability of Trypsin Inhibitor in Foun Bruchid-Resistant Mung Bean Varieties
摘要
Abstract
Taking bruchid-resistant mung beans C6749, C5200, C5193, and C5205 as experimental materials, with a susceptible mung bean Jinlyu 1 as control, the activity and the stability of mung bean trypsin inhibitor were measured under high temperature, high pressure, denaturant and reductant stresses. The trypsin inhibitor activities of four bruchid-resistant mung beans were significantly higher (P<0.01) than those of control (Jinlyu 1), showing C5200 > C5193 > C6749 > C5205. When treated with high temperature, high pressure, denaturant and reductant, the residual activities of trypsin inhibitor from the four bruchid-resistant mung beans were higher than those of control, and decreased with increasing temperature and pressure, and extending treatment time of denaturant and reductant. The effects of denaturant and reductant on the residual activity of trypsin inhibitor of bruchid-resistant mung beans showed a tendency of guanidine hydrochloride > ure, and TCEP > DTT > β-ME. Among the four bruchid-resistant mung beans, C5200 had the highest tolerance and C5193 had the moderate tolerance to high temperature, high pressure, denaturant and reductant stresses; C5205 had the lowest tolerance to high temperature, high pressure, denaturant stresses, while C6749 had the lowest tolerance to reductant treatment. We concluded that C5200 and C5193 have the highest residual activity of bruchid-resistant mung bean trypsin inhibitor under high temperature, high pressure, denaturant and reductant stresses, being of the higher value in its application.关键词
抗豆象绿豆/胰蛋白酶抑制剂/高温高压/变性剂/还原剂Key words
bruchid-resistant mung bean/trypsin inhibitor/high temperature and high pressure/denaturant/reductant引用本文复制引用
樊艳平,成小芳,王宏民,张耀文,张仙红..四个抗豆象绿豆品种的胰蛋白酶抑制剂稳定性[J].作物学报,2018,44(6):867-875,9.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-08-G10)和山西省重点研发计划重点项目(201703D211002-8)资助. The study was supported by the China Agriculture Research System (CARS-08-G10) and the Major Project of Key Research and Develop-ment Program in Shanxi Province (201703D211002-8). （CARS-08-G10）
