作物学报2018，Vol.44Issue(6)：938-946,9.DOI:10.3724/SP.J.1006.2018.00938
基于高密度Bin图谱的水稻抽穗期QTL定位
QTL Mapping for Heading Date in Rice Using High-density Bin Map
摘要
Abstract
A recombination inbred lines (RIL) population including 192 lines derived from an inter-subspecific cross between indica rice 'Yuzhenxiang' and japonica rice '02428' was used in the experiment. The two parent varieties and RIL population were separately sequenced by Whole Genome Sequencing (WGS) and Genotyping-By-Sequencing (GBS) to construct a genetic linkage map with 2711 recombination Bin markers. The number of markers on 12 chromosomes ranged from 162 to 311, and the average physical distance between two markers was 137.68 kb. The WinQTL Cartographer 2.5 was used to analysis QTLs associated with heading date in four different environments. A total of 14 QTLs associated with heading date were detected on chromosomes 1, 2, 3, 7, 8, 9, and 10. Among them, qHD2.2 and qHD10.2, which explained 5.14%-11.15% and 5.35%-16.97% of the total phenotypic variation for heading date separately, could be detected in three environments and shorting heading date about 1.66 days and 1.56 days on average. The two QTLs could inherit stablely, having a good potential to be applied in QTL pyramiding. After comparing the physical positions of these QTLs with those previously reported, we found 11 QTLs were located in the same or near position, among them qHD1.1, qHD2.2, and qHD9.1 were newly reported. Furthermore, we found one cloned gene LOC_Os02g46450 and two annotated genes LOC_Os02g46710 and LOC_Os02g46940 in the genomic region of qHD2.2, might be related to heading date. DNA sequence comparison between YZX and 02428 revealed that all the three genes could be candidate genes.关键词
水稻/抽穗期/Bin图谱/QTL定位Key words
rice/heading date/Bin map/QTL mapping引用本文复制引用
董骥驰,杨靖,郭涛,陈立凯,陈志强,王慧..基于高密度Bin图谱的水稻抽穗期QTL定位[J].作物学报,2018,44(6):938-946,9.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-01-12),国家重点研发计划项目(2016YFD0102102)和广东省应用型研发项目(2015B020231011)资助.This study was supported by the China Agriculture Research System (CARS-01-12), the National Key Research and Development Program of China (2016YFD0102102), and the Research and Development Program for Application in Guangdong Province (2015B020231011). （CARS-01-12）
