重庆医学2018，Vol.47Issue(16)：2117-2121,5.DOI:10.3969/j.issn.1671-8348.2018.16.002
抗人IgM抗体对鼻咽癌HNE-1细胞生物学特性的影响
Effect of anti-human IgM antibody on biological characteristics of human nasopharyngeal carcinoma HNE-1 cell line in vitro and in vivo
摘要
Abstract
Objective To investigate the effect of anti-human immunoglobulin M (IgM) on proliferation,apoptosis,cell cycle and tumor formation in human nasopharyngeal carcinoma HNE-1 cell line in vitro and in vivo.Methods After treatment with anti-human IgM antibody,proliferation of HNE-1 cells was observed by cell proliferation inhibition assay,apoptosis and cell cycle of HNE-1 cells were detected by flow cytometry,and apoptotic cells were detected by TUNEL staining.Nude mouse models were constructed,and were injected intraperitoneally with anti-human IgM antibodies (once every 3 days).The growth of transplanted tumor was observed once every 4 days.After the fifth injection,the expression levels of IgM and gp96 protein in transplanted tumor were observed by immunohistochemical method (streptavidin-peroxidase conjugated method,SP).Results MTS assay showed that anti-human IgM antibody can significantly inhibit the proliferation of HNE-1 cells in concentration-and time-dependent manner (P＜0.05).Flow cytometry showed that the anti-human IgM antibody promoted a significant decrease in percentage of cells in G1 phase,a significant increase in percentage of cells in S phase,and a significant increase in apoptotic rate of HNE-1 cells (P＜0.05).TUNEL staining showed that the anti-human IgM antibody promoted apoptosis of HNE-1 cells (P＜0.01).Transplantation tumor experiment showed that anti-human IgM antibody can significantly inhibit the volume and weight of transplanted tumor (P＜0.05).The immunohistochemistry showed that the expression levels of IgM and gp96 proteins in mouse transplanted tumors after intraperitoneal injection with anti-human IgM antibodies were significantly lower than those of the control group (P＜0.05).Conclusion The anti-human IgM anti-body could effectively inhibit the proliferation of HNE-1 cells,promote apoptosis,and arrest cell cycle.Anti-human IgM antibody could also inhibit the growth of transplanted tumor in nude mouse,which might be related to inhibition of the expressions of IgM and gp96 proteins.关键词
鼻咽肿瘤/免疫球蛋白M/抗体/HNE-1/生物学特性Key words
nasopharyngeal neoplasms/immunoglobulin M/antibody/HNE-1/biological characteristics分类
医药卫生引用本文复制引用
周珊,刘彦婷,赵飞鹏,冯华君,涂晓敏,杨金亮,梁传余,覃纲..抗人IgM抗体对鼻咽癌HNE-1细胞生物学特性的影响[J].重庆医学,2018,47(16):2117-2121,5.基金项目
国家自然科学基金资助项目(81602570,81773529) （81602570,81773529）
泸州市人民政府-西南医科大学科技战略合作项目(2015LZCYD-S02 4/11). （2015LZCYD-S02 4/11）
