动物营养学报2018，Vol.30Issue(2)：515-523,9.DOI:10.3969/j.issn.1006-267x.2018.02.015
亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响
Effects of Flaxseed Oil on Intestinal Mucosa Structure and Immune Cells of Weaned Piglets after Lipopolysaccharide Challenge
摘要
Abstract
The purpose of this study was to investigate the effects of flaxseed oil on intestinal mucosa structure and immune cells of weaned piglets after lipopolysaccharide (LPS) challenge.Twenty-four Duroc×Landrace×Yorkshine crossed-bred weaned piglets with average body weight (BW) of (9.15±0.90) kg were randomly divided into four groups with six replicates each and one pig per replicate.The experiment consisted of a 2×2 factorial design,and the main factors included:1) diet treatment (5％ flaxseed oil or 5％ corn oil);2) immunological challenge (LPS or physiological saline injection).After being fed diets supplemented with 5％ corn oil or 5％ flaxseed oil for 21 days,pigs were injected intraperitoneally with 100 μg/kg BW LPS or the same doze of saline half and half.At 4 h postinjection,pigs were anaesthetized and slaughtered,and the intestinal samples were collected for analysis.The results showed as follows:1) LPS challenge significantly decreased villus height in jejunum and ileum as well as crypt depth in jejunum (P＜0.05),and significantly increased the lamina propria cell density and the number of neutrophils in jejunum (P＜0.05).LPS challenge tended to reduce the number of epithelial lymphocytes (IELs) in jejunum (P=0.080)and the number of goblet cells in ileum (P=0.059).2) LPS challenge did not significantly affect villus height in jejunum and ileum of pigs in flaxseed oil groups (P＞0.05).However,dietary 5％ flaxseed oil tended to increase the Peyer's patch cell density in ileum (P =0.064),especially under LPS challenge,significantly reduced the lamina propria cell density in jejunum (P＜0.05),and tended to increase the number of IELs in jejunum (P =0.069).These results indicate that 5％ flaxseed oil can maintain small intestinal mucosa structure and immune function of weaned piglets after LPS challenge.关键词
亚麻籽油/断奶仔猪/脂多糖/肠黏膜免疫细胞Key words
flaxseed oil/weaned piglets/lipopolysaccharide/intestinal mucosa immune cells分类
农业科技引用本文复制引用
李先根,朱惠玲,涂治骁,王树辉,王海波,张琳,秦琴,汪龙梅,王秀英,刘玉兰..亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响[J].动物营养学报,2018,30(2):515-523,9.基金项目
湖北省教育厅优秀中青年科技创新团队项目(T201508) （T201508）
