动物营养学报2018，Vol.30Issue(2)：570-577,8.DOI:10.3969/j.issn.1006-267x.2018.02.021
持续偏热环境下饲养密度对肉鸡盲肠菌群多样性、挥发性脂肪酸和血清脑肠肽的影响
Effects of Stocking Density on Diversity of Cecal Microflora, Cecal Volatile Fatty Acids and Serum Brain Gut Peptides of Broilers under Moderate Ambient Temperatures
摘要
Abstract
The study was conducted to investigate the effects of stocking density on diversity of cecal microflora,cecal volatile fatty acids and serum brain gut peptides of broilers under moderate ambient temperatures.A total of one hundred and forty-four 22-day-old healthy Arbor Acres broilers with similar body weight were randomly divided into three groups,each group contained six cages and each cage as a replicate.The pre-test period lasted for 7 days and broilers were kept at 21 ℃ temperature and 60％ relative humidity.When broilers were 29-day-old,with the area of single chicken cage was 0.64 m2,the stocking density was 6 birds each cage (half male and half female,group Ⅰ),8 birds each cage (half male and half female,group Ⅱ) and 10 birds each cage (half male and half female,group Ⅲ) respectively under 31 ℃ temperature and 60％ relative humidity,and the trial lasted for 14 days.The results showed as follows:1) the richness and diversity of cecal microflora of broilers were the highest in group Ⅱ under the moderate temperature,and the similarity of cecal microflora between group Ⅰ and group Ⅱ was lower than that between group Ⅰ and group Ⅲ.2) The stocking density in group Ⅱ was beneficial to the growth of cecal Bacteroides vulgatus of broilers under the moderate temperature.3) The influence of stocking density on the volatile fatty acid contents in the cecum of broilers was not significantly different under the moderate temperature (P＞0.05).4) At the 7th day of the experiment,the serum 5-hydroxytryptamine (5-HT) of broilers in group Ⅱ was significantly higher than that in group Ⅰ under the moderate temperature (P＜0.05).However the influence of stocking density on serum contents of vasoactive intestinal peptide (VIP) and substance P (SP) of broilers were not significantly different under the moderate temperature (P＞0.05).In conclusion,the stocking density can change the diversity and structure of cecal microflora as well as serum 5-HT content,but does not affect the contents of volatile fatty acids in the cecum of broilers.关键词
偏热环境/饲养密度/菌群多样性/挥发性脂肪酸/血清脑肠肽/肉鸡Key words
moderate temperature/stocking density/microflora diversity/volatile fatty acid/brain gut peptide/broiler分类
农业科技引用本文复制引用
常双双,马贵达,张敏红,石玉祥,冯京海..持续偏热环境下饲养密度对肉鸡盲肠菌群多样性、挥发性脂肪酸和血清脑肠肽的影响[J].动物营养学报,2018,30(2):570-577,8.基金项目
国家“十三五”重点研发计划课题“肉禽舒适环境的适宜参数及限值研究”(2016YFD0500509) （2016YFD0500509）
动物营养学国家重点实验室自主研究课题“热环境影响肉鸡健康生长的分子机制”(2004DA125184G1609) （2004DA125184G1609）
