动物营养学报2018，Vol.30Issue(2)：658-666,9.DOI:10.3969/j.issn.1006-267x.2018.02.031
丙氨酰-谷氨酰胺缓解Diquat诱导的断奶仔猪氧化损伤的影响
Effects of Alanyl-Glutamine Alleviate Oxidative Damage Induced by Diquat of Weaned Piglets
摘要
Abstract
This experiment was conducted to study the effects of alanyl-glutamine (Ala-Gln) alleviate oxidatiVe damage induced by Diquat of weaned piglets.The method of double-factor design is adopted.Twenty four piglets with health condition and similar parity weaned at 21 days of age were randomly divided into 2 groups with 12 replicates in each group and 1 pig in each replicate.Pigs in two groups were fed control diet and control diet+0.30％ Ala-Gln,respectively.After 7 days pre feeding,based on the prophase feeding,the 24 piglets were randomly divided into 4 groups with 6 replicates in each group and 1 pig in each replicate.The four groups were basal diet group,basal diet+0.3％ Ala-Gln group,basal diet stress group and basal diet+0.3％ Ala-Gln stress group,respectively.Piglet oxidative stress was simulated by intraperitoneal injection of 8 mg/kg BW Diquat,and the unstressed groups were injected with the same volume of sterilized saline.The experiment lasted for 7 days.The results showed as follows:1) piglets under oxidative stress state,compare with the basal diet stress group,dietary supplemented with Ala-Gln significantly increased the contents of glutamine (Gln),glutathione (GSH) and activities of glutathione peroxidase (GSH-Px),total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) in serum (P＜0.05).2) Piglets under normal physiological state and oxidative stress state,compared with corresponding basal diet group and basal diet stress group,dietary supplemented with Ala-Gln significantly increased the GSH-Px activity and T-AOC in jejunum (P＜0.05),and significantly decreased the malondialdehyde (MDA) content (P＜0.05);dietary supplemented with Ala-Gln significantly increased the GSH-Px activity and T-AOC in liver (P＜0.05),and significantly decreased the malondialdehyde (MDA) content (P＜0.05).3) Piglets under oxidative stress state,compare with the basal diet stress group,dietary supplemented with Ala-Gln significantly increased the liver glutathione peroxidase 4 (GPx4) mRNA expression (P＜0.05),and significantly decreased the superoxide dismutase 1 (SOD1) mRNA expression (P＜0.05).These findings indicate that piglets under normal physiological state and oxidative stress state,diets supplemented with Ala-Gln can improve antioxidant capacity and decrease MDA content of piglets,and to alleviate the organism tissue damage of oxidative stress of weaned piglets,especially under oxidative stress state.关键词
氧化应激/Ala-Gln/仔猪/抗氧化能力Key words
oxidative stress/Ala-Gln/piglets/antioxidant capacity分类
农业科技引用本文复制引用
辛向荣,叶亚玲,游金明,贺琴,邓宸玺..丙氨酰-谷氨酰胺缓解Diquat诱导的断奶仔猪氧化损伤的影响[J].动物营养学报,2018,30(2):658-666,9.基金项目
国家自然科学基金项目(31360554) （31360554）
江西省科技落地计划项目(KJLD14027) （KJLD14027）
