动物营养学报2018，Vol.30Issue(2)：717-725,9.DOI:10.3969/j.issn.1006-267x.2018.02.037
葡萄籽原花青素对奶牛瘤胃体外发酵参数及微生物区系的影响
Effects of Grape Seed Procyanidine on Rumen Fermentation Parameters and Microflora of Dairy Cows in Vitro
摘要
Abstract
The objective of this trial was to explore the effects of grape seed procyanidin on rumen fermentation parameters and microflora of dairy cows by in vitro culture method.The trial was divided into 6 groups.Total mixed ration with concentrate to forage ratio at 40 ∶ 60 was used as the fermentation substrate.Grape seed procyanidin was added at levels of 0,0.1,0.2,0.3,0.4 and 0.5 g/kg,respectively.After fermented for 24 h,gas production was recorded,and rumen fermentation parameters and microorganism contents were determined.The results showed that compared with control group:1) the supplementation of 0.4 and 0.5 g/kg grape seed procyanidin significantly decreased the contents of butyric acid and isovalaric acid of fermentation fluid (P＜0.05),however,the supplementation of 0.2 g/kg grape seed procyanidin significantly increased the content of isobutyric acid of fermentation fluid (P＜0.05);2) the supplementation of different levels of grape seed procyanidin significantly increased rumen fluid pH (P＜0.05);3) the supplementation of different levels of grape seed procyanidin could inhibit gas production,and the effect in 0.3 g/kg group was significant (P＜0.05);4) grape seed procyanidin could significantly reduce the contents of protozoa (0.2,0.3,0.4 and 0.5 g/kg groups),methanogens (0.1,0.2,0.4 and 0.5 g/kg groups),butyrivibrio fibrisolvens (0.2,0.3,0.4 and 0.5 g/kg groups) and fibrobacter succunogene (0.1,0.3,0.4 and 0.5 g/kg groups) of fermentation fluid (P＜0.05).In summary,the supplementation of grape seed procyanidin improves rumen fermentation pattern,has a significant effect on rumen microflora,and significantly inhibits methane production;the optimal supplemental level is 0.2 g/kg.关键词
葡萄籽原花青素/奶牛/瘤胃液/体外发酵/微生物区系Key words
grape seed procyanidin/dairy cow/rumen fluid/in vitro fermentation/microflora分类
农业科技引用本文复制引用
杨德莲,童津津,张婕,郭琪,蒋琦晖,蒋林树,熊本海..葡萄籽原花青素对奶牛瘤胃体外发酵参数及微生物区系的影响[J].动物营养学报,2018,30(2):717-725,9.基金项目
国家“十三五”重点研发计划(2016YFD0700201,2016YFD0700205) （2016YFD0700201,2016YFD0700205）
北京市农业局“北京市现代农业产业技术体系奶牛创新团队” （）
