动物营养学报2018，Vol.30Issue(2)：790-796,7.DOI:10.3969/j.issn.1006-267x.2018.02.045
金银花提取物对瘤胃体外发酵参数及产气量的影响
Effects of Honeysuckle Extract on Rumen in Vitro Fermentation Parameters and Gas Production
摘要
Abstract
This experiment was conducted to examine the effects of honeysuckle extract supplementation on rumen in vitro fermentation parameters and gas production.Four healthy Holstein cows fitted with permanent ruminal cannulas were used as the donors of rumen fluid.Honeysuckle extract was added in fermentation substrate of different groups at 0 (control),0.5,1.0,2.0 and 4.0 mg/g,respectively.Each group had 6 replicates,and the test was repeated for three times.Gas production was recorded after fermented for 1.5,3.0,6.0,12.0 and 24.0 h,respectively;rumen fermentation parameters were determined after fermented for 24.0 h.The results showed as follows:1) compared with control group,the supplementation of 1.0,2.0 and 4.0 mg/g honeysuckle extract significantly decreased pH and ammonia nitrogen (NH3-N) concentration in fermentation fluid (P＜0.05),and significantly increased the concentrations of lactic acid,microbial protein (MCP) and total volatile fatty acid,and gas production at 24.0 h (P＜0.05).2) Fermentation fluid pH,and the concentrations of NH3-N,lactic acid and total volatile fatty acid in 0.5 mg/g honeysuckle extract group were not significantly different from those in control group and the other experimental groups (P＞0.05),only MCP concentration and gas production at 24.0 h were significantly higher than those of control group (P＜0.05).The results indicate that the supplementation of honeysuckle extract can effectively regulate the fermentative state of rumen microorganism under the in vitro condition,and considering economic benefit,the optimal supplemental level of honeysuckle extract is 1.0 mg/g.关键词
金银花提取物/瘤胃体外发酵/奶牛Key words
honeysuckle extract/rumen in vitro fermentation/dairy cow分类
农业科技引用本文复制引用
唐志文,蒋林树,杨亮,孙福昱,熊本海..金银花提取物对瘤胃体外发酵参数及产气量的影响[J].动物营养学报,2018,30(2):790-796,7.基金项目
国家“十三五”重点研发课题(2016YFD0700205,2016YFD0700201) （2016YFD0700205,2016YFD0700201）
