动物营养学报2018，Vol.30Issue(3)：981-988,8.DOI:10.3969/j.issn.1006-267x.2018.03.022
葡萄糖对杂交石斑鱼(褐点石斑鱼♀×清水石斑鱼♂)受精卵孵化及卵黄囊仔鱼乙酰辅酶A羧化酶、脂肪酶合成酶活性的影响
Effects of Glucose on Fertilized Eggs Hatch, Activities of Acetyl-CoA Carboxylase and Fatty Acid Synthase of Yolk-Sac Larvae of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus polyphekadion ♂)
摘要
Abstract
In order to investigate the effects of glucose on fertilized eggs hatch,and activities of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) of yolk-sac larvae of hybrid grouper,different concentrations of glucose were added to the water for hatching of hybrid grouper (Epinephelus fuscoguttatus ♀ ×Epinephelus polyphekadion ♂),which were 0,2,4,6,8 and 10 mg/L,respectively.The hatching rate and abnormality rate were observed,and the total length,activities of ACC and FAS of yolk-sac larvae at 1,2 and 3 days of age were tested.The results showed as follows:hatching rate of fertilized eggs and abnormalities of newly hatched larvae were significantly affected by glucose concentration (P＜0.05);the correlation between the concentration of glucose and hatching rate of fertilized eggs and abnormality rate of newly hatched larvae both could be described by quadratic equations.The optimum glucose concentration was 3.8 mg/L to reach the highest hatching rate of fertilized eggs.When the concentration of glucose was below 6 mg/L,hatching rate of fertilized eggs increased,while abnormality rate of newly hatched larvae decreased,total length of yolk-sac larvae increased with the increase of concentration of glucose.For yolk-sac larvae at 1,2 and 3 days of age,activities of ACC and FAS in glucose supplemented group were significantly higher than those in control group (P＜0.05).With the increase of concentration of glucose,the activities of ACC and FAS of larvae at 1 day of age increased;at 2 and 3 days of age,ACC activity of larvae displayed a'up-down'trend and FAS activity showed a'rising-gentle'trend.It is concluded that in artificial breeding of hybrid grouper,the supplementation of 6 mg/L glucose in hatching water can promote fertilized egg hatch and yolk-sac larvae development,and significantly increase ACC and FAS activities of yolk-sac larvae.关键词
葡萄糖/杂交石斑鱼/孵化率/卵黄囊仔鱼/乙酰辅酶A羧化酶/脂肪酶合成酶Key words
glucose/hybrid grouper/hatching rate/yolk-sac larvae/acetyl-CoA carboxylase/fatty acid synthase分类
农业科技引用本文复制引用
黄建盛,阮涛,陈刚,张健东,王忠良,汤保贵..葡萄糖对杂交石斑鱼(褐点石斑鱼♀×清水石斑鱼♂)受精卵孵化及卵黄囊仔鱼乙酰辅酶A羧化酶、脂肪酶合成酶活性的影响[J].动物营养学报,2018,30(3):981-988,8.基金项目
广东省科技厅项目(2016B020201009,2013B090700010) （2016B020201009,2013B090700010）
广东省教育厅高校重点实验室滚动支持项目(2013CXDA019) （2013CXDA019）
广东海洋大学“创新强校工程”项目(GDOU2016050251) （GDOU2016050251）
广东普通高校创新团队项目(2015KCXTD021) （2015KCXTD021）
国家海水鱼产业技术体系专项经费项目(CARS-47-GO8) （CARS-47-GO8）
