动物营养学报2018，Vol.30Issue(3)：989-998,10.DOI:10.3969/j.issn.1006-267x.2018.03.023
发酵豆粕替代鱼粉对黄姑鱼幼鱼生长性能、血清生化指标及肝脏中类胰岛素生长因子-Ⅰ基因表达的影响
Effects of Replacement of Fish Meal by Fermented Soybean Meal on Growth Performance, Serum Biochemical Indices and Liver Insulin-Like Growth Factor-Ⅰ Gene Expression of Juvenile Yellow Drum (Nibea albflora)
摘要
Abstract
An 8-week feed trial was conducted to evaluate the effects of replacement of fish meal by fermented soybean meal on growth performance,serum biochemical indices and liver insulin-like growth factor-Ⅰ (IGF-Ⅰ) gene expression of juvenile yellow drum (Nibea albflora),and to determine the appropriate ratio of fermented soybean meal replacement of fish meal in juvenile yellow drum diet.A basal diet which contained 45％ fish meal was formulated using Peru fish meal,soybean meal and wheat meal as main protein sources,and fish oil,soybean oil and soybean lecithin as main lipid sources.Six isonitrogenous (protein level was 50％) and isolipidic (lipid level was 12％) experimental diets were formulated by replacing 0 (FSM0 group,as control group),10％ (FSM10 group),20％ (FSM20 group),30％ (FSM30 group),40％ (FSM40 group) and 50％ (FSM50 group) fish meal with fermented soybean meal on the basis of the basal diet,respectively.All diets were supplemented with lysine and methionine except the control group diet to keep the lysine and methionine contents consistent in each group.A total of 360 juvenile yellow drum with the initial body weight of (31.24±0.02) g were divided into 6 groups with 3 replicates per group and 20 fish per replicate.The results showed that the weight gain rate,specific growth rate and feed conversion rate of fish in FSM10,FSM20 and FSM30 groups had no significant differences compared with control group (P＞0.05).The weight gain rate and specific growth rate of fish in FSM40 and FSM50 groups were significantly lower than those of fish in control group (P＜0.05),however,the feed conversion rate of fish in FSM40 and FSMS0 groups was significantly higher than that of fish in control group (P＜0.05).No significant differences were observed in the survival ratio,hepatosomatic index,viscerosomatic index and condition factor (P＞0.05).The serum glutaminc oxalacetic transaminase (AST) activity of fish in FSM40 and FSM50 groups was significantly higher than that of fish in other groups (P＜0.05),and the fish in FSM50 group had a significantly higher glutamic-pyruvic transaminase (ALT) activities compared with that of fish in control group and FSM10 group (P＜0.05).The fish in FSMS0 group had a significantly lower IGF-Ⅰ gene relative expression level compared with that of fish in control group (P＜0.05).Overall,The appropriate substitute range of fermented soybean meal replacement of fish meal are 20％ to 30％,high replacement ratio will cause a decrease in the growth performance and feed utilization efficiency of juvenile yellow drum.关键词
黄姑鱼幼鱼/发酵豆粕/鱼粉/生长性能/IGF-Ⅰ基因Key words
juvenile yellow drum (Nibea albflora)/fermented soybean meal/fish meal/growth performance/IGF-Ⅰ gene分类
农业科技引用本文复制引用
王立改,曾文繁,楼宝,詹炜,陈睿毅,刘峰,徐冬冬,谢庆平..发酵豆粕替代鱼粉对黄姑鱼幼鱼生长性能、血清生化指标及肝脏中类胰岛素生长因子-Ⅰ基因表达的影响[J].动物营养学报,2018,30(3):989-998,10.基金项目
国家自然科学基金项目(41476127) （41476127）
浙江省科技厅开展协同创新项目(2016F50038) （2016F50038）
舟山市科技计划项目(2015C31010) （2015C31010）
浙江海洋大学博士启动基金(2014Q1434) （2014Q1434）
