动物营养学报2018，Vol.30Issue(3)：1027-1034,8.DOI:10.3969/j.issn.1006-267x.2018.03.027
呕吐毒素对IPEC-J2细胞凋亡的影响
Effects of Deoxynivalenol on Apoptosis of IPEC-J2 Cells
摘要
Abstract
This experiment was conducted to determine the effect of deoxynivalenol (DON) on the apoptosis of IPEC-J2 cells after different concentrations and time treatment.An appropriate exposure time (6,12,and 24 hour) used CCK-8 assay to test for cell proliferation.The effects of DON on cell mitosis cycle and early cell apoptosis were examined by treated the IPEC-J2 cells with low concentration (200 ng/mL) and high concentrations (2 000 ng/mL) DON for 6,12,24 hours.The results showed as follows:1) DON significantly inhibited the growth of IPEC-J2 cells.At 200 and 2 000 ng/mL DON concentrations,the cell survival rate of 48 and 72 h was significantly lower than that of 24 h (P＜0.05),and the cell survival rate of 72 h was significantly lower than that of 48 at 2 000 ng/mL DON concentration (P＜0.05).2) Different DON concentrations had different effects on cell cycle.The low concentration of DON mainly acted on mitosis stage S and interferes with the replication of DNA,but the high concentration of DON mainly acted on the mitotic phase G2/M and interferes with the synthesis of enzymes and spindle filament proteins.3) DON for 6 hours,the early apoptosis rate and total apoptosis rate of low concentration DON group and high concentration DON group were significantly higher than those of control group (P＜0.05);DON for 12 hours,the early apoptosis rate and total apoptosis rate of high concentration DON group were significantly higher than those of control group and low concentration DON group (P＜0.05).In conclusion,DON can inhibit IPEC-J2 cells proliferation,low concentration of DON can caused cell mitosis to stay in the S phase,high concentration of DON can caused cell mitosis to stay in the G2/M period.DON also can promote cell apoptosis and increase total apoptosis rate,and this effect is enhanced with DON concentration increasing.关键词
呕吐毒素/IPEC-J2细胞/周期/凋亡/增殖Key words
DON/IPEC-J2 cells/cycle/apoptosis/proliferation分类
农业科技引用本文复制引用
廖美芳,孟英才,詹济华,印遇龙,廖鹏,李玲..呕吐毒素对IPEC-J2细胞凋亡的影响[J].动物营养学报,2018,30(3):1027-1034,8.基金项目
国家自然科学基金青年基金(31402088) （31402088）
