动物营养学报2018，Vol.30Issue(3)：1095-1102,8.DOI:10.3969/j.issn.1006-267x.2018.03.034
沙葱黄酮对肉羊生产性能和肠道组织β-防御素基因表达的影响
Effects of Allium mongolicum Regel Flavonoids on Performance and β-Defensins Gene Expression in Intestinal Tissue of Meat Sheep
摘要
Abstract
The objective of this study was to investigate the effects of Allium mongolicum Regel flavonoids on performance and the expression of immune factors-β-defensin 1 (sBD-1) and β-defensin 2 (sBD-2) in intestinal tissue of meat sheep.Single factor completely randomized block design was used in the current study,a total of 60 healthy and six-month-old thin-tailed Han wethers with similar body weight [(39.9±3.2) kg] were randomly divided into 4 groups with 15 sheep each.Sheep in control group were fed a basal diet,and those in experimental groups were fed the basal diet supplemented with 11 (low-dose flavonoids group),22 (mid-dose flavonoids group) and 33 mg/kg Allium mongolicum Regel flavonoids (high-dose flavonoids group),respectively.The trial period lasted for 75 d,which consisted of 15 d of pre-feeding period and 60 d of formal period.Intake was recorded every day and sheep were weighted before feeding every 15 d.At the end of the experiment,three sheep were randomly selected from each group and slaughtered to collect the duodenum,jejunum and ileum tissues.The relative expression levels of genes involved in sBD-1 and sBD-2 were measured by real time-qPCR.The results showed as follows:1) at the 30th to 45th day of experiment,the average daily feed intake of meat sheep in experimental groups was significantly higher than that in control group (P＜0.05),and the highest average daily feed intake was found in high-dose flavonoids group.At the 45th to 60th day of experiment,the average daily gain was significantly increased and the feed to gain ratio was significantly decreased in experimental groups compared with the control group (P＜0.05),and the highest average daily gain and the lowest feed to gain ratio were found in mid-dose flavonoids group and high-dose flavonoids group,respectively.2) Compared with the control group,diet supplemented with 33 mg/kg Allium mongolicum Regel flavonoids significantly increased the relative expression levels of sBD-1 gene in jejunum and ileum and sBD-2 gene in jejunum (P＜0.05),and diet supplemented with 22 mg/kg Allium mongolicum Regel flavonoids significantly increased the relative expression levels of sBD-2 gene in duodenum (P＜0.05).In conclusion,diet supplemented with 33 mg/kg Allium mongolicum Regel flavonoids can significantly increase the performance and the expression of β-defensins (sBD-1 and sBD-2) genes in intestine of meat sheep.关键词
沙葱黄酮/肉羊/生产性能/免疫/β-防御素/基因表达Key words
Allium mongolicum Regel flavonoids/meat sheep/performance/immune/β-defensins/gene expression分类
农业科技引用本文复制引用
陈圣阳,敖长金,郑彦楷,陈仁伟,刘旺景,木其尔,白晨..沙葱黄酮对肉羊生产性能和肠道组织β-防御素基因表达的影响[J].动物营养学报,2018,30(3):1095-1102,8.基金项目
国家自然科学基金(31460611) （31460611）
