动物营养学报2018，Vol.30Issue(6)：2097-2108,12.DOI:10.3969/j.issn.1006-267x.2018.06.012
21日龄断奶对仔猪肠道形态、肠道通透性及肠黏膜屏障的影响
Effects of Weaning at 21 Days of Age on Intestinal Morphology, Permeability and Mucosal Barrier of Piglets
摘要
Abstract
This experiment was conducted to investigate the effects of weaning at 21 days of age on intestinal morphology, permeability and mucosal barrier of piglets. According to 2 × 3 double-factor completely random-ized test design, groups (nursing group and weaning group) and days of age (22, 24 and 28 days of age) as the two main factors. Healthy Yorkshire piglets with similar body condition were selected from 6 litters, and 6 piglets with average body weight of (6.1±0.2) kg were selected from each litter and assigned to 2 groups ( nursing group and weaning group) with 18 piglets per group. Piglets were slaughtered at 22, 24 and 28 days of age, respectively, and growth performance, intestinal morphology, permeability and mucosal barrier were measured. The results showed as follows:1) the final body weight and average daily gain of piglets in weaning group were significantly lower than that in nursing group at 28 days of age ( P<0.01) . 2) There were signifi-cant interactions of group and days of age on crypt depth and the ratio of villus height to crypt depth ( V/C) of jejunum and villus height of ileum of piglets (P<0.01). Crypt depth of jejunum and ileum in weaning group was significantly higher than that in nursing group ( P<0.01) , and villus height and V/C of jejunum and ileum in weaning group were significantly lower than that in nursing group (P<0.01). V/C of jejunum in nursing group at 28 days of age was significantly higher than that at 22 and 24 days of age (P<0.01), and villus height of ileum at 24 days of age was significantly higher than that at 22 days of age ( P<0.01) . 3) There was significant interaction of group and days of age on the activity of diamine oxidase ( DAO) of jejunal mucosa of piglets ( P<0.05) , and the activity of DAO of jejunal and ileal mucosa in weaning group was significantly low-er than that of nursing group ( P<0.05) . 4) There was significant interaction of group and days of age on the mRNA expression of occludin of jejunal mucosa of piglets ( P<0.01) . The mRNA expression of occludin of je-junal mucosa in nursing group and zonuls occludens ( ZO-1 ) of jejunal mucosa increased first and then de-creased with the increasing of days of age ( P<0.05) . The mRNA expression of occludin of jejunal mucosa at 24 days of age and ZO-1 and occludin of ileal mucosa in weaning group were significantly lower than that in nursing group ( P<0.05) , and the mRNA expression of ZO-1 of ileal mucosa at 24 days of age was significant-ly higher than that at 22 days of age ( P<0.05) . 5) There was significant interaction of group and days of age on the mRNA expression of tumor necrosis factor-α (TNF-α) of jejunal mucosa of piglets (P<0.05). The mRNA expression of TNF-α of jejunal mucosa at 24 and 28 days of age in weaning group was significantly higher than that in nursing group (P<0.05), and the mRNA expression of interleukin-10 (IL-10) of jejunal mucosa was significantly lower than that in nursing group ( P<0.05) . The mRNA expression of interleukin-1β( IL-1β) of ileal mucosa was decreased with the increasing of days of age ( P<0.05) . In conclusion, the intes-tinal development of nursing piglets becomes mature from 22 to 28 days of age, but weaning breaks the struc-ture of intestinal epithelial cell of piglets and mRNA expression of tight junction proteins, causes intestinal vil-lus shorted and fell off, intestinal permeability increased, inflammatory response and average daily gain de-creased.关键词
断奶仔猪/肠道形态/肠道通透性/肠黏膜屏障Key words
weaning piglets/intestinal morphology/intestinal permeability/intestinal macosal barrier分类
农业科技引用本文复制引用
夏冰,孟庆石,解竞静,吴维达,冯潇慧,唐湘方,张宏福..21日龄断奶对仔猪肠道形态、肠道通透性及肠黏膜屏障的影响[J].动物营养学报,2018,30(6):2097-2108,12.基金项目
国家自然科学基金(31501960) （31501960）
中国农业科学院创新工程(ASTIP-IAS07) （ASTIP-IAS07）
国家自然科学基金(31672428) （31672428）
