动物营养学报2018，Vol.30Issue(6)：2215-2225,11.DOI:10.3969/j.issn.1006-267x.2018.06.025
长期饥饿后异育银鲫对饲料蛋白质的需求
Requirement of Dietary Protein for Gibel Carp ( Carassius auratus gibelio)Suffered from Long-Term Starvation
摘要
Abstract
This study was conducted to obtain the requirement level of dietary protein for juvenile gibel carp ( Carassius auratus gibelio) suffered from long-term starvation and its adaptive strategies. The juvenile gibel carp [(11.6±0.4) g] were fasted for 90 d under (15.2±2.0) ℃ of water temperature, and then diets with protein levels of 20％ ( P20) , 25％ ( P25) , 30％ ( P30) , 35％ ( P35) and 40％ ( P40) were provided ( each replicate had 3 replicates, and each replicate had 40 carps) , respectively. The re-feeding trial lasted 56 d under (24.4±2.2) ℃ of water temperature. Variations of body measurements, viscera index, body composition and activities of intestinal digestive enzymes and were measured during starvation and re-feeding periods. The re-sults showed as follows:1) after 90 d of starvation, body weight was significantly decreased ( P<0.05) , liver index and intestinal tract length index were significantly decreased ( P<0.05) , activities of intestinal digestive enzymes were significantly decreased (P<0.05), body moisture and ash content were significantly decreased ( P<0.05) , and body crude protein and crude lipid were significantly decreased ( P<0.05) . 2) After re-feed-ing, on day 14, there was no significant difference on body weight among groups (P>0.05); on day 28, body weight of P20 group was significantly lower than that of P30, P35 and P40 groups ( P<0.05);on day 42 and 56, body weight of P35 and P40 groups was significantly higher than that of the other groups ( P<0.05) , and specific growth rate ( SGR) and feed conversion ratio ( FCR) in these two groups were close. 3) On day 56 of re-feeding, body crude protein content of P20 group was significantly lower than that of the other groups (P<0.05), body crude lipid content of P20 and P25 groups was significantly lower than that of the other groups (P<0.05), body ash content of P20 and P25 groups was significantly higher than that of the other groups ( P<0.05) , and body moisture content of P20 and P25 groups was significantly higher than that of P35 and P40 groups (P<0.05). 4) After re-feeding, activities of intestinal digestive enzymes were gradually in-creased; protease activity was increased with the improvement of dietary protein level, P35 and P40 groups were significantly higher than the other groups on day 28 ( P<0.05) , and P40 group was significantly higher than the other groups on day 56 ( P<0.05); amylase activity was decreased with the improvement of dietary protein level, P35 and P40 groups were significantly lower than the other groups on day 28 (P<0.05), and P30, P35 and P40 groups were significantly lower than the other groups on day 56 ( P<0.05) . In conclusion, there are differences in utilization and adaptive process to diets with different dietary protein levels for juvenile gibel carp suffered from long-term starvation;at the early stage of re-feeding, the growth is not affected by di-etary protein level, but high dietary protein level as 35％ to 40％ will promote the growth after recovery of in-gestion and digestive function, and the theoretical optimal dietary protein level is 38.1％.关键词
异育银鲫/长期饥饿/饲料蛋白质水平/特定生长率/饲料系数Key words
gibel carp ( Carassius auratus gibelio )/long-term starvation/dietary protein level/specific growth rate/feed conversion ratio分类
农业科技引用本文复制引用
吴本丽,黄龙,何吉祥,陈静,宋光同,武松..长期饥饿后异育银鲫对饲料蛋白质的需求[J].动物营养学报,2018,30(6):2215-2225,11.基金项目
安徽省农业科学院院长青年创新基金(16B0509) （16B0509）
安徽省农业科学院科技创新团队建设项目(13C0506) （13C0506）
