动物营养学报2018，Vol.30Issue(6)：2281-2293,13.DOI:10.3969/j.issn.1006-267x.2018.06.032
控制摄食对小鼠肝脏及肾上腺胆固醇代谢的影响
Effects of Dietary Restriction on Cholesterol Metabolism in Liver and Adrenal Gland of Mice
摘要
Abstract
This experiment was conducted to study the effects of dietary restriction on cholesterol metabolism in liver and adrenal gland of mice. ICR male mice were used as the study subjects, with adaptive feeding 3 days. After weighing up to 24 to 25 g, they were randomly divided into 5 groups with 6 mice in each group, inclu-ding normal control group (free feeding), 4.0 g feeding group [feed intake: 4.0 g/d per mouse; restriction ratio:88％ of normal feed intake], 3.0 g feeding group [feed intake: 3.0 g/d per mouse; restriction ratio:66％ of normal feed intake] , 2.0 g feeding group [ feed intake:2.0 g/d per mouse; restriction ratio:44％ of normal feed intake] and 1.5 g feeding group [ feed intake:1.5 g/d per mouse; restriction ratio:22％ of nor-mal feed intake] . It was free drinking for all groups. After feeding restriction for 14 days, the mice were exe-cuted, and the heart, liver, kidney, spleen, thymus and testis were weighed and the organic indexes were cal-culated. The total RNA and total protein of hepatic and adrenal tissues were extracted. Then, cholesterol metab-olism related genes mRNA relative expression levels were detected by real-time quantitative PCR technology, and steroidogenic acute regulatory protein ( StAR) relative expression level was assayed by Western blot meth-od, and serum corticosterone content were tested by enzyme-linked immuno sorbent assay ( ELISA) method. The results showed as follows:1) compared with the normal control group, the body weight of mice in dietary restriction groups was lost in different degrees, and the heart, liver, kidney, spleen and thymus of mice in di-etary restriction groups were shrunken in different degrees. 2 ) Compared with the normal control group, the expression of low-density lipoprotein receptor ( Ldlr ) , scavenger receptor class B member 1 ( Scarb1 ) , hydroxymethyl glutaric acyl coenzyme A reductase ( Hmgcr) , hormone-sensitive triglayceride lipase ( Lipe) , apolipoprotein E ( Apoe) , ATP-binding cassette transporter G5 ( Abcg5) , ATP-binding cassette transporter G8 ( Abcg8) , cytochromes P4507A1 ( Cyp7a1) , sterol regulatory element binding protein cleavage active protein ( Scap) , liver X receptor β ( Nr1h2) , peroxisome proliferators-activated receptor α ( Ppara) and peroxisome proliferators-activated receptorγ( Pparg) genes in liver of 3.0 g feeding group, 2.0 g feeding group and 1.5 g feeding group were significantly up-regulated (P<0.05 or P<0.01), while the expression of insulin induced gene 2 ( Insig2) gene was significantly down-regulated ( P<0.05) . 3) Compared with the normal control group, the expression of adrenal cytochromes P45011A1 ( Cyp11a1) and cytochromes P45021A1 ( Cyp21a1) genes in ad-renal gland of 2.0 g feeding group and 1.5 g feeding group were significantly up-regulated ( P<0.05) , the expres-sion of StAR and cytochromes P45011B1 ( Cyp11b1) genes in adrenal gland of 1.5 g feeding group were signifi-cantly up-regulated ( P<0.05 ) , and the expression of cytochromes P45011B2 ( Cyp11b2) in 3.0 g feeding group, 2.0 g feeding group and 1.5 g feeding group was significantly down-regulated ( P<0.05) . Additionally, the adrenal StAR protein expression of all dietary restriction groups was strengthen, and it was strengthen with the feed intake decreasing. In conclusion, the organism metabolism such as spleen, thymus and liver are affected by extreme dieta-ry restriction, and then the hepatic and adrenal cholesterol metabolism and conversion are affected.关键词
控制摄食/小鼠/肝脏/肾上腺/胆固醇代谢Key words
dietary restriction/mice/liver/adrenal gland/cholesterol metabolism分类
医药卫生引用本文复制引用
钱宏梁,潘志强,王晓敏,卢涛,方肇勤..控制摄食对小鼠肝脏及肾上腺胆固醇代谢的影响[J].动物营养学报,2018,30(6):2281-2293,13.基金项目
国家自然科学基金面上项目(81473562) （81473562）
