动物营养学报2018，Vol.30Issue(6)：2337-2345,9.DOI:10.3969/j.issn.1006-267x.2018.06.038
抗菌肽Sublancin与黄芪多糖对小鼠免疫调节作用的比较研究
Comparative Study on Immunomodulatory Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharide of Mice
摘要
Abstract
This experiment was conducted to compare the regulating effect of antimicrobial peptide Sublancin and Astragalus polysaccharide on immune function of mice. Sixty female BALB/c mice at the age of 4 to 6 weeks were randomly assigned into 6 groups with 10 mice per group. Mice in blank control group received sa-line by intragastric administration, mice in challenged group received saline by intragastric administration, mice in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups received 12.0 and 48.0 mg/kg BW Astragalus polysaccharide solution by intragastric administration, respectively, and mice in 1.0 and 2.0 mg/kg BW Sub-lancin groups received 1.0 and 2.0 mg/kg BW Sublancin solution by intragastric administration, respectively. All the mice were administrated orally daily for 7 days, and the dose of each mouse was 0.2 mL. Except blank control group, mice in other 5 groups were challenged with Salmonella typhimurium ( 1 × 109 CFU/mL, 200 μL per mouse) on day 8 ( 24 h after intragastric administration) . Five mice from each group were ran-domly selected 3 and 24 h after challenge and the peripheral blood, spleen and caecum contents were sampled to measure the serum cytokine contents, T lymphocyte subsets CD+4 and CD+8 numbers in splenocyte and Salmo-nella number in intestinal contents, and so on. The results showed as follows: challenged 3 h by Salmonella, compared with the challenged group, the serum interleukin-6 ( IL-6) content in 12.0 and 48.0 mg/kg BW As-tragalus polysaccharide groups and 2.0 mg/kg BW Sublancin group was significantly reduced ( P<0.05); the serum interleukin-10 ( IL-10 ) content in 1. 0 mg/kg BW Sublancin group was significantly increased ( P<0.05); the serum tumor necrosis factor-α ( TNF-α) content in 12.0 and 48.0 mg/kg BW Astragalus polysac-charide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly reduced ( P<0.05) , but the serum interleukin-25 ( IL-25) content was not significantly different ( P>0.05);the serum monocyte chemoattractant protein-1 ( MCP-1) content in 48.0 mg/kg BW Astragalus polysaccharide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); CD+4/CD+8 in splenocyte in 48.0 mg/kg BW Astragalus polysac-charide group, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased ( P<0.05);the Salmonel-la number in intestinal contents in 12. 0 and 48. 0 mg/kg BW Astragalus polysaccharide groups, 1. 0 and 2.0 mg/kg BW Sublancin groups had no significant changes (P>0.05), but had reducing trend. Challenged 24 h by Salmonella, compared with the challenged group, the serum IL-6 content in 2.0 mg/kg BW Sublancin group was significantly reduced ( P<0.05); the serum TNF-α content in 48.0 mg/kg BW Astragalus polysac-charide group and 2.0 mg/kg BW Sublancin group was significantly reduced (P<0.05); the serum MCP-1 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 2.0 mg/kg BW Sublancin group was significantly reduced ( P<0.05) . Moreover, the serum IL-10 content in 12.0 and 48.0 mg/kg BW Astragalus polysaccharide groups, 1.0 and 2.0 mg/kg BW Sublancin groups was significantly increased challenged 24 h by Salmonella compared with blank control group ( P<0.05) . These results indicate that antimicrobial peptide Sublancin and Astragalus polysaccharide have regulating effect on immune function for mice, and the immuno-modulatory effect of antimicrobial peptide Sublancin is more comprehensive than that of Astragalus polysaccha-ride.关键词
抗菌肽/Sublancin/黄芪多糖/小鼠/免疫功能Key words
antimicrobial peptide/Sublancin/Astragalus polysaccharide/mice/immune function分类
农业科技引用本文复制引用
杨天任,王帅,黄烁,尚丽君,于海涛,曾祥芳,谯仕彦..抗菌肽Sublancin与黄芪多糖对小鼠免疫调节作用的比较研究[J].动物营养学报,2018,30(6):2337-2345,9.基金项目
农业产业技术体系北京市生猪创新团队项目 （）
北京市科技计划项目 （）
