大学化学2018，Vol.33Issue(5)：51-55,5.DOI:10.3866/PKU.DXHX201801012
分析化学中"检出限"的物理意义及测定方法探究
Study on the Physical Meaning and Determination Method of"Detection Limit" in Analytical Chemistry
摘要
Abstract
In this paper, we studied the physical meaning, measurement methods and potential problems of detection limit in analytical chemistry. The limitation of classical detection limit was also discussed. We studied the detection of thrombin by fluorescence quenching of Pt nanoparticles, based on the specific binding of thrombin aptamer to thrombin. We found a negative correlation between the measured signal and the logarithm of the concentration. In addition, the similar negative correlation was found in the determination of glucose by a glassy carbon electrode biosensor, based on CuGeO3 nanowires and glucose oxidase. The detection limit of the above two method conflicted with the classical detection limits. It appeared abnormal that the better the determination precision, the worse the detection limit.关键词
检出限/物理意义/测定方法Key words
Detection limit/Physical meaning/Determination分类
社会科学引用本文复制引用
杨淳,郑姗,刘洋,施娅颖,黄朝表..分析化学中"检出限"的物理意义及测定方法探究[J].大学化学,2018,33(5):51-55,5.基金项目
国家自然科学基金面上项目(21575129) （21575129）
