广西医科大学学报2018，Vol.35Issue(6)：751-755,5.DOI:10.16190/j.cnki.45-1211/r.2018.06.003
弹性蛋白肽对髓样树突状细胞表面共刺激分子表达及炎症因子分泌的影响
Differences in expression of cell surface co-stimulatory molecules and secretion of inflammatory factors by bone marrow-derived dendritic cells in response to elastin peptide
摘要
Abstract
Objective:To observe the differences in expression of cell surface co-stimulatory molecules and secretion of inflammatory factors by bone marrow-derived dendritic cells(DCs) in response to elastin peptide(EP).Methods:We cultured bone marrow-derived DCs from BALB/c mice and infected with EP.The activation of mveloid DCs(mDCs) was characterized by studying expression of cell surface co-stimulatory molecules(CD80 and CD86),release of transforming growth factor (TGF)-β1 and interleukin (IL)-12.Results:Flow cytometry analysis and qPCR detection showed significant up-regulation of CD80 and CD86 after treatment with EP(P＜0.05).Meanwhile,ELISA revealed that the IL-6 and TGF-β1 levels in cell supernatant was conspicuous elevated(P＜0.05).Conclusion:EP could promote the activation of mDCs in mice and the release of related pro-inflammatory factors.关键词
弹性蛋白肽/树突状细胞/表面共刺激分子Key words
elastin peptide/dendritic cells/surface co-stimulatory molecules分类
医药卫生引用本文复制引用
马婷婷,谭彩梅,许海光,唐艳萍,张慧,张建全..弹性蛋白肽对髓样树突状细胞表面共刺激分子表达及炎症因子分泌的影响[J].广西医科大学学报,2018,35(6):751-755,5.基金项目
国家自然科学基金资助项目(No.81460009) （No.81460009）
广西自然科学基金资助项目(No.2015GXNSFAA139189) （No.2015GXNSFAA139189）
