广西医科大学学报2018，Vol.35Issue(6)：756-761,6.DOI:10.16190/j.cnki.45-1211/r.2018.06.004
自噬在可溶性尿酸诱导肾小管上皮细胞炎症反应中的作用
The role of autophagy in soluble uric acid-induced inflammatory response in renal tubular epithelial cells
摘要
Abstract
Objective:To investigate the role of autophagy in soluble uric acid (UA)-induced inflammatory response in renal tubular epithelial cells (HK-2 cells).Methods:Cells were pretreated with or without UA in soluble form and then stimulated with rapamycin (RAP,an autophagy enhancer) or chloroquine (CQ,an autophagy inhibitor).The changes of autophagosomes were observed under transmission electron microscopy.The expressions of monocyte chemoattractant protein-1 (MCP-1),autophagic microtubule-associated light chain protein 3 (LC3-Ⅱ) and autophagy-related protein Beclin1 were determined by western blotting.Subsequently,HK-2 cells were co-stimulated with soluble UA and an Akt inhibitor perifosine.The expressions of phosphor-Akt (p-Akt) and MCP-1 were detected by western blotting as well.Results:Soluble UA treatment re sulted in up-regulation of MCP-1,LC3-Ⅱ,and Beclin1 in HK-2 cells (P＜0.05) and an increase in the number of autophagosomes.The LC3-Ⅱ and Beclin1 expressions were enhanced while the MCP-1 expression was down-regulated in HK-2 cells after stimulation with RAP (P＜0.05),but these changes were reversed by CQ stimulation (P＜0.05).The expression level of MCP-1 was decreased after stimulation of p-Akt inhibitor perifosine compared with non-treated control (P＜0.05).Conclusion:Autophagy might be involved in the soluble UA-induced inflammation in renal tubular epithelial cells.关键词
自噬/可溶性尿酸/肾小管上皮细胞/炎症Key words
autophagy/soluble uric acid/tubular epithelial cells/inflammation分类
医药卫生引用本文复制引用
陈园园,易扬,覃廖缓,刘福岗,谢恺庆,杨海波..自噬在可溶性尿酸诱导肾小管上皮细胞炎症反应中的作用[J].广西医科大学学报,2018,35(6):756-761,6.基金项目
国家自然科学基金资助项目(No.81360243) （No.81360243）
广西自然科学基金资助项目(No.2013GXNSFAA019149) （No.2013GXNSFAA019149）
