广西医科大学学报2018，Vol.35Issue(6)：761-765,5.DOI:10.16190/j.cnki.45-1211/r.2018.06.005
香烟烟雾暴露小鼠中CD4+Foxp3+调节性T细胞中组蛋白去乙酰化酶的表达及意义
The expression and significance of histone deacetylase in CD4+ Foxp3+ regulatory T cells in cigarette smoke-exposure mice
摘要
Abstract
Objective:To evaluate the expression of histone deacetylase(HDAC) in CD4+ Foxp3+ regulatory T(Treg) cells and the function in cigarette smoke-induced emphysema mice.Methods:40 male Balb/c mice were randomly divided into control group,trichostatin A(TSA,a HDAC inhibitor) group,smoke exposure (SE) group and SE+TSA group,with 10 mice in each group.Morphological changes of lung tissues were observed by hematoxylin eosin(HE) staining.The mRNA expressions of HDAC2,HDAC5,HDAC7 and HDAC9 were determined by qPCR.The expression of cytotoxic T lymphocyte associated antigen-4(CTLA-4) in Treg cells,the proliferation ability of Treg cells and its inhibitory effect on the Teff cells proliferation were detected by flow cytometry.Results:The mean linear intercepts(MLI) was higher in the SE group than that in the control group(P＜0.05),suggesting that the mice emphysema model was successfully established.The HDAC2,HDAC5,HDAC7 and HDAC9 mRNA levels and the Teff cells proliferation rate were significantly decreased,while the positive expression rate of CTLA-4 in the Treg cells and the proliferation rate of Treg cells were increased in SE group and TSA group,compared with the control group(P＜0.05).The positive rate of CT-LA-4 expression,the proliferation ability of Treg cells and its inhibitory effect on the Teff cells proliferation were enhanced by the treatment of TSA in SE mice (P ＜ 0.05).Conclusion:The expression of HDAC in CD4+ Foxp3+ Treg cells was down-regulated in cigarette smoke-induced emphysema mice.HDAC might be involved in the immune imbalance of Treg cells induced by smoking.关键词
调节性T细胞/组蛋白去乙酰化酶/香烟/肺气肿Key words
regulatory T cells/histone deacetylase/smoke/pulmonary emphysema分类
医药卫生引用本文复制引用
李煜梅,杨霞,邱诗林,白晶..香烟烟雾暴露小鼠中CD4+Foxp3+调节性T细胞中组蛋白去乙酰化酶的表达及意义[J].广西医科大学学报,2018,35(6):761-765,5.基金项目
国家自然科学基金资助项目(No.81260011) （No.81260011）
广西自然科学基金资助项目(No.2013jjAA40109) （No.2013jjAA40109）
