广西医科大学学报2018，Vol.35Issue(6)：774-778,5.DOI:10.16190/j.cnki.45-1211/r.2018.06.008
一种卵巢癌快速药敏试验方法的效果研究
Study on the effect of a fast drug susceptibility test for primary ovarian cancer cells
摘要
Abstract
Objective:To establish a fast method for determining the sensitivity of various common-used chemotherapy drugs in ovarian cancer patients and evaluate its application value of individualized chemotherapy for ovarian cancer.Methods:Primary tumor cells from the peritoneal metastases tissues of a primary surgery ovarian cancer patient were cultured.Low passage (P2-P4) cell susceptibility to common-used chemotherapy drugs such as paclitaxel,docetaxel,cisplatin,carboplatin,nedaplatin alone or their combination were detected.The sensitization effect of metformin was observed.Results:The normalized cell index (NCI) curve of the platinum group decreased rapidly,and the NCI value was significantly lower than that of other single drugs at 24 h,36 h,48 h and 60 h (P＜0.05).The NCI value of the nedaplatin+docetaxel group at 24 h,36 h and 48 h were significantly lower than that of other two groups (P＜0.05).The NCI value of metformin combination groups were significantly lower than those of the non-compatibility groups at 12 h,48 h and 60 h (P＜0.05).Conclusion:This original peritoneal metastasis ovarian cancer cells were most sensitive to nedaplatin and the combination of nedaplatin and docetaxel,and the combination used of metformin could increase the sensitivity to chemotherapeutic drugs.Analyzing the drug sensitivity of the low passage primary peritoneal metastases cells by RTCA system could provide support for reasonable selection of clinical chemotherapies and judge whether patients could get benefits from the combination used of metformin.关键词
卵巢癌/腹膜转移/原代培养/个体化/奈达铂Key words
ovarian cancer/peritoneal metastasis/primary culture/individualization/nedaplatin分类
医药卫生引用本文复制引用
刘萤照,冯雁,刘红梅,吴健勇,唐勇,王琪..一种卵巢癌快速药敏试验方法的效果研究[J].广西医科大学学报,2018,35(6):774-778,5.基金项目
国家自然科学基金资助项目(No.81360341) （No.81360341）
广西自然科学基金项目(No.2015GXNSFAA139167) （No.2015GXNSFAA139167）
区域性高发肿瘤早期防治研究教育部重点实验室自主研究基金(No.GKE2015-ZZ14) （No.GKE2015-ZZ14）
广西区域性高发肿瘤早期防治研究重点实验室自主研究基金(No.GK2014-ZZ18) （No.GK2014-ZZ18）
