河南农业科学2018，Vol.47Issue(4)：104-108,5.DOI:10.15933/j.cnki.1004-3268.2018.04.019
低温胁迫下菊花叶片叶绿素荧光特性与抗氧化酶活性的变化
Chlorophyll Fluorescence Characteristics and Antioxidant Enzyme Activities of Chrysanthemum Leaves under Low Temperature Stress
摘要
Abstract
Autumn chrysanthemum late-flower cultivar Jinglongtengyun was used as experimental material.The effects of different temperature on antioxidant enzyme activities and chlorophyll fluorescence parameters were studied in order to explore mechanisms in response to low temperature.The results are as followes:in contrast with CK,under 8,4,-4 and-8 ℃ treatments for 12 h the chlorophyll a + b content in leaves decreased by 14.6％,23.2％,45.7％ and 49.9％ respectively and the soluble sugar content increased by 16.5％,19.4％,19.7％ and 23.2％.Under 8 ℃ and 4 ℃ treatments,the trends of Fv/Fm,Fv/Fo and qP were not obvious,but NPQ increased at the early stage.With the increase of treatment time,the trend of NPQ was not obvious at 8 ℃ and decreased slowly at 4 ℃.Under-4 ℃ and -8 ℃ treatments,Fv/Fm,Fv/Fo,qP and NPQ decreased significantly,qP and NPQ were close to zero under-8 ℃ treatment for 12 h.Under 8 ℃ and 4 ℃ treatments,SOD and POD activities increased significantly at 3 hours after treatment,CAT activity decreased at 3 h and then increased.SOD and POD activities increased firstly and then decreased,and CAT activity appeared declining trend under-4 ℃ and -8 ℃ treatments.Under low temperature for 12 h,the MDA content in leaves increased by 11.9％,17.3％,54.2％ and 60.7％.In sunmary,autumn chrysanthemum could relieve low temperature stress by regulating antioxidant enzyme activity,content of soluble sugar,and increasing heat dissipation.However,fluorescence parameters and antioxidant enzyme activity in leaves of chrysanthemum decreased significantly at-8 ℃,and the photosynthetic system was seriously damaged,leading to the loss of photosynthetic capability.关键词
菊花/低温胁迫/抗氧化酶活性/叶绿素荧光Key words
Chrysanthemum/Low temperature stress/Antioxidant enzyme activity/Chlorophyll fluorescence分类
农业科技引用本文复制引用
程祥飞,王磊,聂林杰,李永华..低温胁迫下菊花叶片叶绿素荧光特性与抗氧化酶活性的变化[J].河南农业科学,2018,47(4):104-108,5.基金项目
河南省科技攻关项目(142102110046) （142102110046）
