河南农业科学2018，Vol.47Issue(4)：113-118,144,7.DOI:10.15933/j.cnki.1004-3268.2018.04.021
自然变温下小叶蚊母树叶片生理指标及组织结构变化
Physiological Indexes and Tissue Anatomical Structures of Distylium buxifolium Leaf under Natural Temperature Changes
摘要
Abstract
Aimed to reveal the cold tolerance and adaptive mechanism in leaf of Distylium buxifolium during natural temperature changes in Qingdao,leaves of D.buxifolium were used as materials.The leaf tissue structure,cell cytoplasm membrane permeation and physiological changes of D.buxifolium were investigated by routine paraffin section and determination of relative conductivity and relevant physiological indexes during natural temperature changes in Qingdao.Experimental results showed that the leaf tissue structure and physiological metabolism activities were affected in different degrees with the temperature changed.From October 2015 to April 2016,the temperature reduced at first and then increased.Different indexes of D.buxifolium leaf including relative conductivity,SOD activity,MDA content,soluble protein content and proline content were analyzed,which tend to increase first and then decrease with the temperature.The indexes of leaf thickness and ratio of cell length and width in palisade displayed significantly higher in January 2016 than that in October 2015 and April 2016,while relative volume percentage ratio and cell volume of palisade tissue showed an opposite trend with lower in April 2016.The above results indicate that improved ability of cold tolerance in D.buxifolium relies on the changes of leaf tissue structure and physiological metabolism activities.关键词
小叶蚊母树/相对电导率/生理指标/叶组织结构Key words
Distylium buxifolium/Relative conductivity/Physiological indexes/Leaf tissue structure分类
农业科技引用本文复制引用
姜新强,李璐璐,刘庆超,刘庆华,王奎玲..自然变温下小叶蚊母树叶片生理指标及组织结构变化[J].河南农业科学,2018,47(4):113-118,144,7.基金项目
青岛市民生科技计划项目(14-2-3-36-nsh) （14-2-3-36-nsh）
