江西农业大学学报2018，Vol.40Issue(3)：429-434,6.DOI:10.13836/j.jjau.2018057
水稻花药开裂前茉莉酸水平及信号途径相关基因表达的动态变化
The Dynamic Changes in Jasmonate Levels and Expression of its Pathway?Related Genes in Anthers Before Dehiscence in Rice
摘要
Abstract
Anther dehiscence is a critical step for reproduction of flowering plants.Abnormal anther dehis?cence will directly result in poor pollination and fertilization.Rice anther dehiscence is synchronized with floret opening which is caused by swelling of lodicules.Phytohormone jasmonate (JA) is an important signaling mole?cule in regulating anther dehiscence and floret opening.In this study,the dynamic changes in endogenous JA levels and expression of JA pathway genes in anthers 24?0 h before splitting were doserved to investigate the reg?ulation of JA signaling before rice anther dehiscence.The result showed that the levels of JA and active conjugate jasmonoyl?isoleucine (JA?Ile) in anthers increased sharply from 2 h before the splitting.The JA and JA?Ile lev?els in splitting anthers were respectively 8.3 and 55 times than in ones of 2 h before splitting.Concurrently,the expression levels of OsLOX6,OsAOS1,OsAOC and OsOPR7 which encode enzymes involved in JA biosynthesis pathway were up?regulated partly from 24 to 0 h before anther dehiscence.The expression of OsJAR1 did not in?creased obviously and OsJAR2 decreased rapidly befor eanther dehiscence;these two homologous genes encode JA?Ile synthetase.The expression levels of OsCOI1a,OsCOI1b and OsCOI2 which encode JA signaling receptors were down?regulated slightly.However,the expression levels of OsJAZ10 and OsJAZ11,acting as repressors of JA signaling,increased 65 and 85 times from 24 to 0 h before anther dehiscence. These results indicated that the increase of JA level and differential activation of genes related in JA signaling pathway was closely correla?ted with the dehiscence program of rice anther.关键词
水稻/花药开裂/茉莉酸/JAZ蛋白Key words
rice/anther dehiscence/jasmonate/JAZ proteins分类
农业科技引用本文复制引用
何永明,刘遂飞,雷抒情..水稻花药开裂前茉莉酸水平及信号途径相关基因表达的动态变化[J].江西农业大学学报,2018,40(3):429-434,6.基金项目
国家自然科学基金项目( 31360295)和江西省青年科学基金项目( 20151BAB214014) Project supported by the National Natural Science Foundation of China (31360295) and Youth Science Founda?tion of Jiangxi Province (20151BAB214014) （ 31360295）
