江西农业大学学报2018，Vol.40Issue(3)：606-611,6.DOI:10.13836/j.jjau.2018078
丁酸对瘤胃上皮细胞生长和凋亡的影响
Effect of Butyric Acid On Growth and Apoptosis of Rumen Epithelial Cells
摘要
Abstract
The aim of this study was to investigate the effect of butyric acid on the growth and apoptosis of rumen epithelial cells in vitro. Rumen epithelial cells were treated with different concentrations of butyrate acid (0,20,40,60,80,100,120,140 mmol/L) and different treatment time (4,6,8,10 h).After that,the viability of epithelial cells in each group was detected by MTT assay. Then the suitable treatment group was used to detect cell apoptosis by TUNEL assay,DNA ladder assay and flow cytometry. The results showed that:with the increase of butyric acid concentration,the relative cell viability of rumen epithelial cells increased firstly,and then decreased.Additive of 20 mmol/L butyric acid promoted the growth of rumen epithelial cells, while 40-140 mmol/L butyrate decreased the viability of ruminal epithelial cells.Apoptosis detection revealed that apoptosis of rumen epithelial cells induced by the additive of 120 mmol/L butyric acid cultured for 6 h. The total apoptosis index of epithelial cells in 120 mmol/L butyrate group was 28.4％,which was significantly higher than that in the control group (24.1％,P<0.05).In conclusion,low concentration of butyric acid promo?ted the growth rate of rumen epithelial cells,while high concentration of butyric acid induced the apoptosis of rumen epithelial cells.关键词
丁酸/瘤胃上皮细胞/细胞活力/细胞凋亡Key words
butyric acid/rumen epithelial cells/cell viability/apoptosis分类
农业科技引用本文复制引用
彭志鹏,罗丹,许超,熊小文,温庆琪,瞿明仁,欧阳克蕙..丁酸对瘤胃上皮细胞生长和凋亡的影响[J].江西农业大学学报,2018,40(3):606-611,6.基金项目
国家自然科学基金项目(31560648,31260561)、公益性行业(农业)科研专项项目(201303143?04)和国家星火计划重点项目( 2015GA730003) Project supported by National Natural Science Foundation of China (31560648,31260561),Special Fund for Ag?ro?scientific Research in the Public Interest ( 201303143?04 ) and Key Program of China Spark Program ( 2015GA730003) （31560648,31260561）
