临床口腔医学杂志2018，Vol.34Issue(6)：326-329,4.DOI:10.3969/j.issn.1003-1634.2018.06.003
实时定量PCR结合DGGE检测慢性牙周炎患者不同生境中的牙龈卟啉单胞菌和齿垢密螺旋体
The quantity of Porphyromonas gingivalis and Treponema denticola in the three habitat environment of the chronic periodontitis by using real-time PCR cooperated with DGGE
摘要
Abstract
Objective:To detect Porphyromonas gingivalis and Treponema denticola in the different habitats of pa-tients with chronic periodontitis by using real-time PCR combined with denaturing gradient gel electrophoresis ( DGGE ) . Methods:Thirty chronic periodontitis volunteers were selected to collect supragingival plaque,subgingival plaque and saliva samples. The microbial community structure of each group was analyzed by DGGE. Then the related dominant bands in differ-ent habitats could be found through partial least squares( PLS) . By means of cloning and sequencing analyzing them,the nea-rest bacteria could be determined. In addition,the quantitative detection and analysis of Porphyromonas gingivalis and Trepo-nema denticola between supra and subgingival plaque,subgingival plaque and saliva was illustrated by using real-time poly-merase chain reaction( PCR) . Results:It is showed that the quantity of Porphyromonas gingivalis and Treponema denticola in subgingival plaque was higher than in supragingival plaque and saliva by using real-time PCR technique. Conclusion:Real-time PCR cooperated with DGGE can interpret the microbial community information better.关键词
实时定量PCR/变性梯度凝胶电泳技术/牙周炎/牙龈卟啉单胞菌/齿垢密螺旋体Key words
Real-time PCR/Denaturing gradient gel electrophoresis(DGGE)/Periodontitis/Porphyromonas gingi-valis/Treponema denticola分类
医药卫生引用本文复制引用
邹岩,刘莹,唐子圣,漆正楠,尹君,王畅,吴明桃..实时定量PCR结合DGGE检测慢性牙周炎患者不同生境中的牙龈卟啉单胞菌和齿垢密螺旋体[J].临床口腔医学杂志,2018,34(6):326-329,4.基金项目
国家重点研发计划项目(2017YFC0840100,2017YFC0840110) （2017YFC0840100,2017YFC0840110）
中国科学院重大科技基础设施开放研究项目 （）
上海交通大学"医工(理)交叉研究"基金资助项目(YG2013MS58) （理）
