生态与农村环境学报2018，Vol.34Issue(5)：448-455,8.DOI:10.11934/j.issn.1673-4831.2018.05.009
有色金属矿区植物根际耐镉菌的分离鉴定与镉吸附特性
Isolation, Identification and Adsorption Characteristics of Cd-Resistant Rhizobacteria in a Nonferrous Metal Mine
摘要
Abstract
Four dominant plant species and their rhizosphere soil samples were collected from the nonferrous metal mine of Baoshan,Hunan Province.The plant species were identified as Dianthus superbus,Caryopteris incana var.incana,Dendranthema indicum and Patrinia villosa.Concentrations of Cd in the plants and soil were determined,and it was found that Dendranthema indicum and Dianthus superbus had the stronger ability to absorb Cd from soil than Caryopteris incana var.incana and Patrinia villosa.Due to its high-metal accumulation capacity,Dendranthema indicum and Dianthus superbus showed a potential ability to apply in artificial ecological restoration system.Based on morphology,RDP-Ⅱ identification system and 16S rDNA gene sequence analysis,three Cd-resistant strains of bacteria (BS1,BS2,BS3) isolated from the rhizospheric soil were finally confirmed as Sphingomonas echinoides,Massiliaflava and Bacillus aryabhattai.Their minimal inhibitory concentration levels against cadmium were up to 300,100,80 mg · L-1,respectively.Three factors,including temperature,adsorption time and pH may influence their Cd2+ absorption ability.The results show that,under the condition of initial concentration of 50 mg · L-1 Cd exposure,the optimal growth temperature of three bacterial strains was 30 ℃,and their biosorption amounts of Cd2+ increased slowly with time going on.The adsorption reached saturation when the strains were cultured for 30 to 36 h.Strain BS2 exhibited higher Cd adsorption rates of 57.32％ and 54.15％ under the condition of pH 5.5 and 6.5 than others.The Cd2+ adsorption rate of strain BS1 reached 18.83％ to 29.14％,under three pH conditions.Strain BS3 showed the maximum adsorption rate of 40.66％ only at pH 6.5.关键词
有色金属矿区/镉/根际细菌/吸附Key words
nonferrous mine/cadmium/rhizosphere bacteria/adsorption分类
资源环境引用本文复制引用
汪婵娟,熊治廷,徐仲瑞,刘荣相..有色金属矿区植物根际耐镉菌的分离鉴定与镉吸附特性[J].生态与农村环境学报,2018,34(5):448-455,8.基金项目
国家重点研发计划(2017YFD0801502) （2017YFD0801502）
