山东医药2018，Vol.58Issue(21)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.21.001
HIV-1感染对人CD4+T淋巴母细胞内ITAMs/ITIMs通路的影响
Effects Of HIV-1 infection on related genes and proteins of ITAMs / ITIMs signaling pathway in human CD4 + T lymphoblast cells
摘要
Abstract
Objective To investigate the expression of related genes and proteins of immunoreceptortyrosine-based ac-tivation motifs(ITAMs)/ immunoreceptortyrosine-based inhibition motifs (ITIMs)signaling pathway in human CD4 + T lym-phoblast cells (M8166 cells)after HIV-1 infection. Methods M8166 cells cultured in vitro were randomly divided into the blank group and infection group. The cells in the blank group were normally cultured without special treatment,while the cells in the infection group were infected with 10 TCID50 HIV-1. The mRNA expression levels of related genes of ITAMs/ ITIMs signaling pathway were detected by real-time fluorescent quantitative PCR,and the expression of the protein of related receptors was detected by Western blotting. Results Compared with the blank group,the expression of SHP-1 mRNA in the M8166 cells increased after HIV-1 infection (P < 0. 01),and the expression of SHIP-1 mRNA showed a de-creasing trend but there was no statistical significance (P > 0. 05). No significant difference was found in the mRNA ex-pression of SHP-2,SHIP2,Zap70,and Syk (all P > 0. 05). On the other hand,the expression of SHP-1 and P-SHP-1 protein significantly increased after HIV-1 infection (both P < 0. 05),and the expression of SHIP1 and P-SHIP1 proteins significantly decreased (both P < 0. 05). The expression of Syk was slightly up-regulated and P-Syk was down-regulated, but there was no statistical difference (both P > 0. 05). No significant difference was found in the expression of SHP-2, SHIP2 and P-SHIP2 between these two groups (all P > 0. 05),and no expression was found in P-SHP2,Zap70,and P-Zap70. Conclusion The ITAMs/ ITIMs signaling pathway of M8166 cells is activated after HIV-1 infection.关键词
获得性免疫缺陷综合征/人免疫缺陷病毒1/人CD4+T淋巴母细胞/M8166细胞/免疫受体酪氨酸激活基序/免疫受体酪氨酸抑制基序/免疫调控通路Key words
acquired immunodeficiency syndrome/human immunodeficiency virus-1/human CD4 + T lymphoblast cells/M8166 cells/immunoreceptortyrosine-based activation motifs/immunoreceptortyrosine-based inhibition motifs/im-mune regulatory pathway分类
医药卫生引用本文复制引用
莫雨晓,谢延峥,刘京,郭晓强,何金洋..HIV-1感染对人CD4+T淋巴母细胞内ITAMs/ITIMs通路的影响[J].山东医药,2018,58(21):1-4,4.基金项目
国家自然科学基金资助项目(81573861). （81573861）
