山东医药2018，Vol.58Issue(21)：13-16,4.DOI:10.3969/j.issn.1002-266X.2018.21.004
帕瑞昔布预处理对肺缺血再灌注损伤大鼠的保护作用及机制
Protective effect of parecoxib preconditioning on lung ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effect of parecoxib preconditioning on lung ischemia-reperfusion injury (LIRI)in rats and its mechanism. Methods Thirty-two male SD rats were randomly divided into four groups (n =8):sham operation group (group S),ischemia-reperfusion group (group I),parecoxib group (group P),and zinc proto-porphyrin group (group Z). Unilateral LIRI model was established by blocking the hilum of the left lung. In the group I, the hilum of the left lung was dissociated and blocked. In the group P,15 minutes before the left lung hilum was blocked , parecoxib 10 mg/ kg was injected via the femoral vein. In the group Z,24 hours before ischemia ,Zinc protoporphyrin 20 mg/ kg was injected intraperitoneally. In addition,an equal volume of saline was injected via the femoral vein in the group S and group I. Arterial blood gas was measured at 5 min before hilar was blocked (T sion. Oxygenation index (OI)and alveolar arterial oxygen pressure difference (PA-aO weight ratio (W/ D ratio)was calculated. The pathological changes of lung tissues were observed. The expression of heme oxygenase 1 (HO-1)mRNA and protein in the lung tissues were detected by RT-PCR and Western blotting. Results At T1 ,compared with group S,OI decreased and PA-aO and those were more significant in the group I and group Z (both P < 0. 05). Compared with group S,the lung W/ D ratio was higher in the other three groups (P < 0. 05),and lung W/ D ratio in the group I and group Z was higher than that in the group P (P < 0. 05). After lung ischemia-reperfusion,the lung tissues of group I,group P,and group Z were signifi-cantly damaged,but the group P was less than the other two groups. Compared with group S,the expression of HO-1 mR-NA and protein in the group I,group P,and group Z increased,with the highest increase in group P (all P < 0. 05);there 0 )and 120 min (T 1 )after reperfu-2 )were calculated;lung wet/ dry 2 increased in the group I,group P,and group Z (both P < 0. 05),was no significant difference between group I and group Z (P > 0. 05)Conclusion Parecoxib pretreatment can improve lung oxygenation and reduce pulmonary edema after LIRI in rats by up-regulating the HO-1 expression in the lung tissues.关键词
肺缺血再灌注损伤/帕瑞昔布/血红素加氧酶1/大鼠Key words
lung ischemia-reperfusion injury/parecoxib/heme oxygenase-1/rats分类
医药卫生引用本文复制引用
吕强,李美亭,刘宇,胡云霞..帕瑞昔布预处理对肺缺血再灌注损伤大鼠的保护作用及机制[J].山东医药,2018,58(21):13-16,4.基金项目
四川省卫生厅科研项目(110233). （110233）
