山东医药2018，Vol.58Issue(21)：17-20,4.DOI:10.3969/j.issn.1002-266X.2018.21.005
食管鳞状细胞癌组织中miR-21、 PDCD4表达变化及意义
Expression changes and significance of miR-21 and PDCD4 in esophageal squamous-cell carcinoma tissues
摘要
Abstract
Objective To observe the expression changes of miR-21 and programmed cell death 4 (PDCD4)in the e-sophageal squamous-cell carcinoma tissues and to explore the clinical significance. Methods The real-time PCR was em-ployed to detect the mRNA expression of miR-21 and PDCD4 in the esophageal squamous-cell carcinoma tissues and adja-cent normal tissues. According to the average of the expression,the patients with the esophageal squamous cell carcinoma were divided into the high-miR-21 group and the low-miR-21 group,or the high-PDCD4 group and the low-PDCD4 group. The correlation between the miR-21 and PDCD4 in the esophageal squamous-cell carcinoma tissues was analyzed by the Spearman. The Kaplan Meier and the Log Rank were employed to detect the influence of miR-21 and PDCD4 expressions on the prognosis. Results The relative expression level of miR-21 in the esophageal squamous-cell carcinoma tissues was higher than that in the adjacent normal tissues,while the mRNA level of PDCD4 in the esophageal squamous-cell carcinoma tissues was lower than that in the adjacent normal tissues,and there were significant difference (both P < 0. 05). The Spearman results showed that the miR-21 and PDCD4 was negatively correlated in the esophageal squamous-cell carcinoma tissues (r = - 0. 883,P = 0. 000). The 5-year survival rate in the high-miR-21 group was lower than that in the lower-miR-21 group (χ2 = 4. 720,P = 0. 030),the same was the survival time (P = 0. 029). The 5-year survival rate in the high-PDCD4 group was higher than that in the low-PDCD4 group (χ2 = 7. 871,P = 0. 005),the same was the survival time (P = 0. 006). Conclusions In the esophageal squamous-cell carcinoma tissues,the miR-21 is highly expressed and the PDCD4 is low expressed,both of which may be involved in the occurrence and development of esophageal squamous-cell carcinoma,and have important influence on the prognosis.关键词
食管鳞状细胞癌/微小RNA-21/程序性细胞死亡基因4/预后Key words
esophageal squamous-cell carcinoma/microRNA-21/programmed cell death 4/prognosis分类
医药卫生引用本文复制引用
马光耀,孙大强,商宏伟,周方..食管鳞状细胞癌组织中miR-21、 PDCD4表达变化及意义[J].山东医药,2018,58(21):17-20,4.基金项目
天津市自然科学基金资助项目(16JCYBJC40502). （16JCYBJC40502）
