色谱2018，Vol.36Issue(6)：518-522,5.DOI:10.3724/SP.J.1123.2018.03017
QuEChERS-超高效液相色谱-串联质谱法测定 动物源食品中痕量五氯酚及其钠盐
Determination of trace pentachlorophenol and its sodium salt in animal-origin foods by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of trace pentachlorophenol and its sodium salt in animal-origin foods by modified QuEChERS-ultra performance liquid chromatog-raphy-tandem mass spectrometry (UPLC-MS/MS ). Sodium pentachlorophenolate in samples was converted to pentachlorophenol under acidic condition. The pentachlorophenol was extrac-ted twice with acetonitrile containing 1% (v/v ) acetic acid by ultrasonic extraction. The extracts were purified by dispersive solid-phase extraction. The usages of dispersive sorbents were optimized based on the recoveries and matrix effects. Chromatographic analysis was con-ducted on a Waters Acquity UPLC HSS T3 column with gradient elution. The pentachlorophenol was further analyzed by negative electrospray ionization under the multiple reaction monitoring mode. The recoveries at fortification levels of 1. 0,2. 0 and 10. 0 μg/kg in six matrices (pork, pork liver,chicken,fish,milk and egg)ranged from 73. 2% to 108. 4% with the relative stand-ard deviations of 4. 0%-14. 8%. The limits of quantification (S/N>10)were 1. 0 μg/kg. The method is simple,sensitive,accurate,economical and environmentally safe,and is suitable for the determination of the trace pentachlorophenol and its sodium salt in animal-origin foods.关键词
超高效液相色谱-串联质谱/QuEChERS/五氯酚/五氯酚钠/动物源食品Key words
ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/QuEChERS/pentachlorophenol/sodium pentachlorophenolate/animal-origin foods分类
化学化工引用本文复制引用
王连珠,方恩华,王彩娟,陈泳,林子旭,徐敦明..QuEChERS-超高效液相色谱-串联质谱法测定 动物源食品中痕量五氯酚及其钠盐[J].色谱,2018,36(6):518-522,5.基金项目
福建省自然科学基金项目(2011J01386,2016J01070).Natural Science Foundation of Fujian Province (Nos.2011J01386,2016J01070). （2011J01386,2016J01070）
