色谱2018，Vol.36Issue(6)：547-551,5.DOI:10.3724/SP.J.1123.2017.09043
气相色谱-三重四极杆质谱法快速检测鸡蛋中氟虫腈及其代谢物
Quick determination of fipronil and its metabolites in eggs by gas chromatography-triple quadrupole mass spectrometry
邓武剑 1吴卫东 2赵旭 2梁宏 2梁淑雯 2靳保辉 2王申1
作者信息
- 1. 赛默飞世尔科技(中国)有限公司,上海 200120
- 2. 深圳出入境检验检疫局,广东深圳 518000
- 折叠
摘要
Abstract
A method was established for the simultaneous determination of fipronil and its metabolites in eggs by gas chromatography-tandem mass spectrometry coupled with QuECh-ERS. Fipronil and its metabolites were extracted from eggs with acetonitrile,and the solution was dehydrated with 150 mg anhydrous magnesium sulfate. The extracts were purified with 50 mg N-propyl ethylenediamine (PSA)and 50 mg C18,and a capillary column pesticide Ⅱ was used. The analytes were detected in timed selected reaction monitoring (timed-SRM )mode, and were quantified using external standard method with matrix correction standard curves. A good linearity in the range of 1. 0-200 μg/L with correlation coefficients (R2 )>0. 999 was observed. The limits of quantification (LOQs)were between 0. 5 and 1. 0 μg/kg. The recoveries at three spiked levels (2. 0,5. 0 and 10. 0 μg/kg)were in the range of 87. 8% to 111. 5% with RSDs between 2. 0% and 9. 2%(n=3). This new method satisfied the related regulations of the European Union for the determination of fipronil and its metabolites in eggs.关键词
气相色谱-三重四极杆质谱/QuEChERS/氟虫腈及其代谢物/鸡蛋Key words
gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS)/QuECh-ERS/fipronil and its metabolites/eggs分类
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邓武剑,吴卫东,赵旭,梁宏,梁淑雯,靳保辉,王申..气相色谱-三重四极杆质谱法快速检测鸡蛋中氟虫腈及其代谢物[J].色谱,2018,36(6):547-551,5.
