生殖医学杂志2018，Vol.27Issue(6)：566-572,7.DOI:10.3969/j.issn.1004-3845.2018.06.013
甘丙肽及其受体在孕期应激大鼠新生子代前额叶皮层和海马脑区表达的性别差异
Gender difference of galanin and its receptors expression in prefrontal cortex and hippocampus in prenatal stress rat offspring
摘要
Abstract
Objective:To investigate the effects of prenatal stress on the development of the prefrontal cortex (PFC)and hippocampus of the offspring of SD rats.Methods:Twelve SD female rats were randomly split into control and pregnancy stress group.In pregnancy stress group,the stress model of the rats was established(2 hours per day of restraint stress during 8-21 days of pregnancy).The weight of descendant on the day of birth and the number of male and female ratio were recorded.Six male and six female offspring for each group were killed to collect PFC and hippocampus.The expression of galanin (GAL),galanin receptor (GALRs),brain-derived neurotrophic factor(BDNF)and tyrosine kinase B(TrkB)in PFC and hippocampus were detected using Q-PCR method,and the differences between the two groups of offspring were analyzed.Results:Compared to control group,the birth weight of the offspring in the prenatal stress group was significantly decreased[(6.45 ± 0.19) vs.(7.57 ± 0.23) g] (P ＜ 0.05).The ratio of female offspring in prenatal stress group had an increasing trend,but the difference was not significant (P＞0.05).In male descendants,the expression of TrkB in the PFC was significantly increased compared with the control group[(1.35 ±0.12)vs.(1.00 ±0.07)](P＜0.05).In the female descendants,the expressions of GALR1 mRNA[(0.51 ± 0.13) vs.(1.04± 0.11)] and BDNF mRNA[(0.73±0.07)vs.(1.11±0.10)] in the PFC were significantly decreased compared with the control group(P＜0.05).The expression of GAL mRNA in hippocampus was significantly increased[(2.51±0.18)vs.(1.00±0.11)](P＜0.05),and the expression of BDNF mRNA was significantly decreased[(0.75±0.05)vs.(1.00±0.03)] compared with the control group(P＜0.05).Conclusions:Prenatal stress can affect the brain development of offspring rats.Female offspring were more susceptible to pregnancy stress than male.关键词
甘丙肽/甘丙肽受体/孕期应激/子代Key words
Galanin/Galanin receptor/Prenatal stress/Offspring引用本文复制引用
徐涛,董柯梦,王彤,王文娇,李慧,石翠格,杨予涛,徐志卿..甘丙肽及其受体在孕期应激大鼠新生子代前额叶皮层和海马脑区表达的性别差异[J].生殖医学杂志,2018,27(6):566-572,7.基金项目
国家自然科学基金面上项目(81671345) （81671345）
北京市自然科学基金重点项目(KZ201610025026) （KZ201610025026）
中央级公益科研院所基本科研业务费专项项目(2017GJM02) （2017GJM02）
