生殖医学杂志2018，Vol.27Issue(6)：573-578,6.DOI:10.3969/j.issn.1004-3845.2018.06.014
亚砷酸钠对胚胎p66Shc表达调控的机制研究
Molecular mechanism of arsenite-mediated regulation of p66Shc expression
摘要
Abstract
Objective:To explore the molecular mechanism of arsenite-mediated regulation of embryonic p66Shc expression.Methods:After 5 μmol/L sodium arsenite treatment,the transcriptional level of p66Shc was detected by real-time quantitative PCR analysis and the expression of Sirt1 was detected by Western blot.The cells were transfected with Sirt1 or control siRNA.At 24 hours post-transfection,cells were exposed to 5 μmol/L sodium arsenite for another 24 hours,and proteins were extracted for Western blot analysis to detect the effect of sodium arsenite on the expression of Sirt1 and p66Shc proteins.Results:The expression of p66Shc on transcriptional level was significantly increased in sodium arsenite treated-group compared with the control group(P＜0.001).Expression of Sirt1 was significantly decreased in sodium arsenite treated-group compared with the control group(P＜0.001).Transfection of Sirt1 siRNA could repress the expression of Sirt1.After sodium arsenite treatment,Sirt1 expression was significantly decreased in Sirt1 siRNA transfection group compared with control siRNA transfection group (P＜0.001),while p66Shc expression was significantly increased (P＜0.001).Conclusions:Sodium arsenite may up-regulate p66Shc expression by inhibiting the expression of Sirt1.关键词
亚砷酸钠/Sirt1/p66Shc/氧化应激Key words
Sodium arsenite/Sirt1/p66Shc/Oxidative stress引用本文复制引用
高小博,张辰,郑盼盼,骆海燕,马旭,陆彩玲..亚砷酸钠对胚胎p66Shc表达调控的机制研究[J].生殖医学杂志,2018,27(6):573-578,6.基金项目
国家自然科学基金项目(81470525) （81470525）
