苏州科技大学学报（自然科学版）2018，Vol.35Issue(2)：45-54,10.DOI:10.12084/j.issn.2096-3289.2018.02.010
产IAA菌的筛选、鉴定及其培养基的优化
Selection, identification of an IAA biosynthesis strain and the optimization of its culture medium
摘要
Abstract
We obtained purified single bacterial strains through separation by dilute plate method from the soil of the garden,and through submerged cultivation screened out a high-yield IAA (29.127 mg·L -1) bacterium GMZB-12, which was proved to be Klebsiella oxytoca through germ detector and 16S rDNA authentication. On the basis of the influence of growth of high-yield IAA bacterium GMZB-12 through tests on single-factor culturing conditions including incubation time,carbon source and nitrogen source,the incubation condition of high-yield IAA bacterium was optimized through the response surface methodology. The best incubation condition was confirmed to be with sucrose concentration of 10 g·L-1,yeast power concentration of 11.2 g·L-1,MgSO4 concentration of 0.22 g·L-1 and CaCl2 concentration of 0.22 g·L-1. the seed germination test shows that GMZB-12 bacterial suspension has the function of promoting the growth of branch paddy roots.关键词
促生菌/吲哚乙酸/响应面实验/促生能力Key words
plant growth-promoting rhizobacteria/IAA/response surface methodology/promoting ability分类
农业科技引用本文复制引用
徐婧,邵锴,李东芳,周捷,钱玮,邱业先..产IAA菌的筛选、鉴定及其培养基的优化[J].苏州科技大学学报（自然科学版）,2018,35(2):45-54,10.基金项目
苏州市环保局项目(B20130503) （B20130503）
苏州市科技项目(SYN201414) （SYN201414）
