天津科技大学学报2018，Vol.33Issue(2)：13-19,7.DOI:10.13364/j.issn.1672-6510.20160224
TMEPAI蛋白表达对溶酶体稳定性的影响
Effect of TMEPAI Protein Expression on Lysosome Stability
摘要
Abstract
The purpose of the research is to generate stable cell lines overexpressing TMEPAI and then study the effect of TMEPAI expression on lysosome stability.The recombined expression vector,pEF-IRES-TMEPAI-Flag plasmid,was constructed with genetic engineering technology.The expression plasmid was positively identified with restriction enzyme digestion,PCR detection and DNA sequencing.A549 lung cancer cells were transfected with pEF-IRES-TMEPAI-Flag plasmid.Stable cell lines were generated after puromycin screening,and verified by Western blot and immunofluorescence.Furthermore,MTT assay,flow cytometry and AO staining assays indicated that the expression of TMEPAI increased the stability of lysosome.关键词
TMEPAI/溶酶体稳定性/氯喹/吖啶橙Key words
TMEPAI/lysosome stability/chloroquine/acridine orange分类
生物科学引用本文复制引用
罗深恒,白喜龙,景磊,李玉银,刁爱坡..TMEPAI蛋白表达对溶酶体稳定性的影响[J].天津科技大学学报,2018,33(2):13-19,7.基金项目
国家自然科学基金资助项目(31471335) （31471335）
