天津中医药2018，Vol.35Issue(6)：466-469,4.DOI:10.11656/j.issn.1672-1519.2018.06.17
柚皮苷通过Fas膜受体通路调控髓核细胞凋亡的机制研究
Mechanism of naringin on the apoptosis of nucleus pulposus cells through Fas membrane receptor pathway
摘要
Abstract
[Objective] To study the effects of naringin on the apoptosis of nucleus pulposus cells of human degenerative intervertebral disc. [Methods] Nucleus pulposus cells were cultured in vitro and were identified via the safranin O and toluidine blue. Next, P3 cells were cultured with naringin 0 g/mL (control group), naringin group (20 g/mL, 40 g/mL, 80 g/mL). After 48 hours, the best concentration of naringin was selected by MTT for inhibition apoptosis of human degenerated nucleus pulposus cells and the rate of apoptosis in each group was measured by flow cytometry. [Results] The results of cells staining were positive, and it was proved that the cells cultured in the experiment were nucleus pulposus cells. MTT and flow cytometry were used to determine the optimum concentration of naringin (20 g/mL) to inhibit the apoptosis of nucleus pulposus cells(P<0.05). Naringin(20 g/mL) could inhibit the expression of Fas, FasL, Caspase-8 mR-NA by qRT-PCR (P<0.05). Western-blot detection showed that 20 g/mL naringin could inhibit the expression of Fas, FasL, Caspase-8 pro-tein. [Conclusion] Naringin may inhibit the apoptosis of nucleus pulposus cells by regulating the Fas/FasL death receptor apoptosis pathway.关键词
柚皮苷/人髓核细胞/凋亡/椎间盘/退变Key words
naringin/human nucleus pulpous/apoptosis/intervertebral disc/degeneration分类
医药卫生引用本文复制引用
苑珍珍,杨召,金鸿宾,许海委..柚皮苷通过Fas膜受体通路调控髓核细胞凋亡的机制研究[J].天津中医药,2018,35(6):466-469,4.基金项目
国家自然科学基金项目(31500781). （31500781）
