中国组织工程研究2018，Vol.22Issue(14)：2209-2214,6.DOI:10.3969/j.issn.2095-4344.0737
可自主结合转化生长因子β1聚乙二醇水凝胶的制备及其生物相容性
Preparation and biocompatibility of polyethylene glycol hydrogels auto-recruiting transforming growth factor beta1
摘要
Abstract
BACKGROUND:Studies have shown that bioactive molecules or polypeptides grafted onto the surface of polyethylene glycol (PEG) hydrogels can improve PEG bioactivities.OBJECTIVE:To manufacture PEG hydrogels capable of auto-recruiting growth factor beta1 (TGF-β1) and to study its biocompatibility. METHODS:Pure PEG hydrogels (group A), PEG hydrogels grafted on a cell adhesion peptide RGD peptides (group B), PEG hydrogels grafted with auto-recruited TGF-β1 peptide sensitive polypeptide HSNGLPL (group C), PEG hydrogels grafted with both RGD and HSNGLPL polypeptides (group D) were prepared. Contract angle of the hydrogel was detected in each group. Human bone mesenchymal stem cells were seeded onto four kinds of hydrogels. After cells attached, scanning electron microscope and LIVE/DEAD staining were done to observe cell-hydrogel compounds. Human bone marrow mesenchymal stem cells were co-cultured with ordinary culture medium (control) or four kinds of hydrogels for 1, 3, 5, 7 days, and the cell proliferation was detected by cell counting kit-8 assay. The four kinds of hydrogels were put into 24-well culture plates with addition of PBS containing TGF-β1, and 1 hour later, immunofluorescence staining was done. RESULTS AND CONCLUSION:(1) The contact angles of groups A and C were larger than those of groups B and D. (2) Under the scanning electron microscope, groups A and C had little cells attached on the hydrogel surface, but there were many cells on the hydrogel surface in groups B and D. (3) LIVE/DEAD staining showed groups A and C had little living cells, and conversely groups B and D had many living cells. (4) The results of cell counting kit-8 demonstrated that as the incubation time went on, cell proliferation activity of five different groups increased with no difference at the same time point. (5) Findings from the immunofluorescence staining showed that groups A and B had very weak fluorescence, while groups C and D had stronger green fluorescence. In conclusion, PEG hydrogels grafted with RGD and HSNGLPL polypeptides can auto-recruit TGF-β1, and have good biocompatibility.关键词
水凝胶/生物相容性/聚乙二醇/转化生长因子β1/转化生长因子β1吸附多肽/生物材料/细胞黏附多肽RGD分类
医药卫生引用本文复制引用
鞠晓晶,潘国庆,刘星志,孙树金,黄立新,施勤..可自主结合转化生长因子β1聚乙二醇水凝胶的制备及其生物相容性[J].中国组织工程研究,2018,22(14):2209-2214,6.基金项目
国家自然科学基金面上项目(81101369) Funding:the National Natural Science Foundation of China, No. 81101369 （81101369）
