畜牧与兽医2018，Vol.50Issue(6)：1-6,6.
基于GC/TOF-MS技术研究猪健康大卵泡和中等卵泡液的代谢变化
Differences in the metabolomics signatures of porcine follicular fluids collected from large and medium sized follicles based on the GC/TOF-MS technique
摘要
Abstract
To characterize the composition of follicular fluids (FF) collected from large and medium sized follicles of domestic pigs,gas chromatography coupled to time-of-flight mass spectrometry (GC/TOF-MS) was used to detect endogenous metabolites in the three-breed crossbred porcine follicular fluids collected from follicles of large (5-8 mm) and medium (3-5 mm) diameters.Finally,metabolite profiles of the follicular fluids were determined by multivariate statistical analysis,such as principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA).The result showed that the metabolic profiles of the large FF and the medium FF were evidently different.The concentrations of 33 metabolites differed from each other due to different follicle sizes (P＜0.05).The concentrations of all these metabolites,except glucose,α-ketoglutaric acid,proline,and glucose l-phosphate,were lower in the large FF,compared with the medium FF;which was mainly associated with the TCA cycle,glycometabolism,amino acid and nucleoside metabolism,based on the biochemical metabolic pathway analysis.There was a distinct shift in follicular metabolic pathway as follicles increased in diameter.Our findings might provide a new clue for study on developmental potential of the oocytes,early embryo development,and regulation of follicular development by means of artificial intervention.关键词
卵泡发育/代谢组学/差异代谢物/代谢通路Key words
follicular development/metabonomics/differential metabolites/metabolic pathway分类
农业科技引用本文复制引用
程玲,王文绍,索川,马志禹,李俊,安娜,雷治海,苏娟..基于GC/TOF-MS技术研究猪健康大卵泡和中等卵泡液的代谢变化[J].畜牧与兽医,2018,50(6):1-6,6.基金项目
教育部留学归国人员启动基金(090600591) （090600591）
