畜牧与兽医2018，Vol.50Issue(6)：19-24,6.
利用高效液相色谱仪测定猪乳中乳清蛋白含量的方法及其应用
Determination of whey protein in sow milk by HPLC and application of the method
摘要
Abstract
This study was to establish a rapid and precise method with high-performance liquid chromatography (HPLC) for determination of lysozyme,lactoferrin,α-lactalbumin,serum albumin and β-1actoglobulin.Chromatographic columns such as Supelcosil C18 (250 mm × 4.6mm,5 μm) and Xbridge TM Sneild RP C18 (150 mm × 4.6 mm,5 μm) were used separately.The chromatographic separation was performed using the following eluents:solvent A,0.1％ trifluoroacetic acid (TFA) in a solution of acetonitrile (ACN)-H2O (5:95);and solvent B,0.1％ TFA in ACN-H2O (95:5).The results showed that the five proteins could be well separated.The calibration curves were linear in the ranges of 25-1 000 mg · L-1 with all the correlation coefficients higher than 0.99 and the relative standard deviations lower than 5％,the recoveries of the five proteins were in the ranges of 91％-97％.The detection limits of lysozyme,lactoferrin,α-lactalbumin,serum albumin and β-lactoglobulin were 0.2,4.0,0.6,0.3 and 1.0 mg · L-1,respectively.The whey proteins in sow milk were determined using this method.The results showed that the contents of β-lactoglobulin,lysozyme,lactoferrin and serum albumin decreased gradually within 16 days after the parturition,the contents of them were the highest on the first day and were 17.2,0.224,2.0 and 15.6 g · L-1,respectively.The contents of β-lactoglobulin,lysozyme,lactoferrin and serum albumin were the lowest on the sixteenth day after parturition and were 5.6,0.092,0.5 and 3.6 g · L-1,respectively.The content of α-lactalbumin increased gradually after parturition,with the highest value of about 3.3 g · L-1 on the seventh day;however,the content of α-lactalbumin decreased to and remained at 3.1 g · L-1.In conclusion,the HPLC method here to determine whey proteins could effectively detect the contents of whey proteins in sow milk.关键词
高效液相色谱/猪乳/乳清蛋白Key words
HPLC/sow milk/whey proteins分类
农业科技引用本文复制引用
杨怀荣,田时祎,赵道远,胡平,王珏,汪晶,吕波..利用高效液相色谱仪测定猪乳中乳清蛋白含量的方法及其应用[J].畜牧与兽医,2018,50(6):19-24,6.基金项目
中央高校自主创新项目(KYZ201722) （KYZ201722）
