畜牧与兽医2018，Vol.50Issue(6)：62-67,6.
山羊重组IL-17A克隆表达与生物活性分析
Cloning, expression and biological activity analysis of recombinant IL-17A in goat
摘要
Abstract
To analyze the effect of goat interleukin 17A on the function of goat monocytes and neutrophils,the IL-17A gene of goat was cloned by RT-PCR and the recombinant protein (rIL-17A) was expressed in vitro.Here,the immuno-modulatory effects of rIL-17A protein on monocyte and neutrophil migration and neutrophil apoptosis were investigated by co-incubation of rIL-17A at different concentrations (5,10,20,40 μg/mL) with monocytes and neutrophils.The results showed that rIL-17Aat the concentrations of 5,20,40 μg/mL significantly enhanced the migration of goat monocytes (P＜0.01),and rIL-17A at the concentration of 10 μg/mL also significantly promoted migration of goat monocytes (P＜0.05).In co-incubation with neutrophils,rIL-17A at the concentrations of 10 (P＜0.05),20 and 40 μg/mL (P＜0.001) significantly promoted migration of goat neutrophils.rIL-17A at the concentrations of 20 μg/mL (P＜0.05) and 40 μg/mL (P＜ 0.001) significantly induced apoptosis of goat neutrophils.All these results suggested that the recombinant IL-17A protein had good biological activity and contributed to migration of goat monocytes,neutrophils and to apoptosis of goat neutrophils to varying degrees in vitro.关键词
白介素17A/单核细胞/中性粒细胞/山羊Key words
IL-17A/monocytes/neutrophils/goat分类
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王雯娟,王强强,田晓薇,陆明敏,严若峰,徐立新,宋小凯,李祥瑞..山羊重组IL-17A克隆表达与生物活性分析[J].畜牧与兽医,2018,50(6):62-67,6.基金项目
国家重点基础发展研究计划(973计划)(2015CB150300) （973计划）
