畜牧与兽医2018，Vol.50Issue(6)：85-89,5.
牦牛感染牛病毒性腹泻病毒的诊断与病毒分离鉴定
Diagnosis of bovine viral diarrhea virus infection in yak by isolation and identification of the virus
马文瑞 1赵庆奎 1李丰清 1张生祥2
作者信息
- 1. 青海省海北州门源县北山乡畜牧兽医站,青海门源810300
- 2. 青海省海北州门源县东川镇克图畜牧兽医站,青海门源810300
- 折叠
摘要
Abstract
To explore the agent of morbid yak calves on yak farms and elucidate its biological traits,clinical diagnosis and virus isolation were performed in this study.The E0 gene of virus was amplified and sequenced,which was then used to analyze homology and construct a phylogenetic tree.The clinical signs of the yak calves were fever,anorexia,oral ulcer,diarrhea,stool with blood and intestinal mucosa and other typical symptoms of BVDV infection.Then,MDBK cells were used to incubate virus in and isolate it from nasal mucus and fecal samples which resulted in obvious cytopathic effect.The titer of the isolated virus was as high as TCID5o at 10-5.19/0.1 mL,which was neutralized by BVDV positive serum and tested to be positive by indirect immunofluorescence detection kit.The E0 nucleotide sequence of the virus showed 99.5％ homology with the subtype BVDV-1b deposited in GenBank.Our results proved the epidemic on the yak farms was BVDV in fection.The virus strain isolated here provided reference for effective prevention and control BVDV infection of yak calves,added to the resources of BVDV strains and molecular epidemiology in China.关键词
牦牛/牛病毒性腹泻病毒/感染/临床诊断/病毒分离鉴定Key words
yak/bovine viral diarrhea virus/infection/clinical diagnosis/virus isolation and identification分类
农业科技引用本文复制引用
马文瑞,赵庆奎,李丰清,张生祥..牦牛感染牛病毒性腹泻病毒的诊断与病毒分离鉴定[J].畜牧与兽医,2018,50(6):85-89,5.
